Division site selection linked to inherited cell surface wave troughs in mycobacteria
Division site selection linked to inherited cell surface wave troughs in mycobacteria
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DOI：
10.1038/nmicrobiol.2017.94
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发表时间：
2017-09-01
影响因子：
28.3
通讯作者：
Fantner, Georg E.
中科院分区：
文献类型：
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作者：
Eskandarian, Haig A.;Odermatt, Pascal D.;Fantner, Georg E.
细胞分裂在空间和时间上受到严格控制，以将细胞大小和倍性保持在狭窄的范围内。在细菌中，典型的微型细胞(Min)和类核封闭(Noc)系统共同确保在完成染色体分离后分裂仅限于中细胞[1]。目前尚不清楚在缺乏Min和Noc蛋白同源物的细菌中，包括导致结核病和其他慢性感染的分枝杆菌中，分裂位点的选择是如何控制的。在这里，我们使用相关的光学和原子力显微镜(3，4)来证明分枝杆菌细胞起伏表面上的形态标志(波形槽)对应于未来的细胞分裂地点。新生细胞继承(大)母细胞的波谷，最终在最中心的波谷分裂，使这些形态特征成为未来分裂地点的最早已知标志。在缺乏染色体分割(PAR)系统的细胞中，染色体的错误分离伴随着偏离中心波谷的不对称细胞分裂，导致无核细胞的形成。这些结果表明，遗传的形态标志和染色体定位共同限制了分枝杆菌分裂到中细胞位置。
Cell division is tightly controlled in space and time to maintain cell size and ploidy within narrow bounds. In bacteria, the canonical Minicell (Min) and nucleoid occlusion (Noc) systems together ensure that division is restricted to midcell after completion of chromosome segregation(1). It is unknown how division site selection is controlled in bacteria that lack homologues of the Min and Noc proteins, including mycobacteria responsible for tuberculosis and other chronic infections(2). Here, we use correlated optical and atomic-force microscopy(3,4) to demonstrate that morphological landmarks (waveform troughs) on the undulating surface of mycobacterial cells correspond to future sites of cell division. Newborn cells inherit wave troughs from the (grand) mother cell and ultimately divide at the centre-most wave trough, making these morphological features the earliest known landmark of future division sites. In cells lacking the chromosome partitioning (Par) system, missegregation of chromosomes is accompanied by asymmetric cell division at off-centre wave troughs, resulting in the formation of anucleate cells. These results demonstrate that inherited morphological landmarks and chromosome positioning together restrict mycobacterial division to the midcell position.