Allergenicity and cross-reactivity of booklice (Liposcelis bostrichophila) : a common household insect pest in Japan
Allergenicity and cross-reactivity of booklice (Liposcelis bostrichophila) : a common household insect pest in Japan
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书虱（Liposcelis bostrichophila）的过敏性和交叉反应性：日本常见的家庭害虫
DOI：
10.1159/000329853
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Fukutomi.Y
中科院分区：
文献类型：
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作者：
Fukutomi Y;Taniguchi M;Tsuburai T,Tanimoto H;Oshikata C;Ono E;Sekiya K;Higashi N;Mori A;Hasegawa M;Nakamura H;Akiyama K;三浦和彦;楢崎幸範;Hosoda S;三浦和彦;Fukutomi.Y
研究背景：书虱（Liposcelis bostrichophila）是一种常见的家庭害虫，分布于世界各地。特别是在日本，它们出没于“榻榻米”垫子，是所有可检测到的昆虫中最常检测到的昆虫，在灰尘样本中出现的频率约为 90%。尽管人们假设它们是一种重要的室内过敏原，但对其过敏性的研究仍然有限。 方法：为了阐明书虱的过敏原性以及这种昆虫过敏原与其他昆虫过敏原的交叉反应性，通过皮肤试验和 IgE-ELISA 从 185 名患有过敏性哮喘的日本成年人中鉴定出对书虱敏感的患者。使用这些患者的血清进行 IgE 抑制分析、免疫印迹和免疫印迹抑制分析。通过二维电泳和二维免疫印迹鉴定了对书虱有特异性致敏作用的过敏蛋白。结果：在 41 名患者（占研究患者的 22%）的血清中检测到了书虱特异性 IgE 抗体。 IgE 抑制分析表明，在本研究中，三分之一书虱过敏患者血清中对书虱过敏原的 IgE 反应性并未因与任何其他环境昆虫提取物预孵育而受到抑制。免疫印迹法从书虱提取物中鉴定出一种 26-kD 的蛋白质，该蛋白质是对书虱产生特异性致敏作用的过敏性蛋白质。该蛋白肽片段的氨基酸序列与之前描述的过敏蛋白没有同源性，表明该蛋白是一种新的过敏原。 结论：该人群对书虱的过敏相对常见，且对该昆虫的特异性过敏与昆虫全过敏无关。
Background:Booklice(Liposcelis bostrichophila)are a common household insect pest distributed worldwide. Particularly in Japan, they infest ‘tatami’ mats and are the most frequently detected insect among all detectable insects, present at a frequency of about 90% in dust samples. Although it has been hypothesized that they are an important indoor allergen, studies on their allergenicity have been limited.Methods:To clarify the allergenicity of booklice and the cross-reactivity of this insect allergen with allergens of other insects, patients sensitized to booklice were identified from 185 Japanese adults with allergic asthma using skin tests and IgE-ELISA. IgE-inhibition analysis, immunoblotting and immunoblotting-inhibition analysis were performed using sera from these patients. Allergenic proteins contributing to specific sensitization to booklice were identified by two-dimensional electrophoresis and two-dimensional immunoblotting.Results:The booklouse-specific IgE antibody was detected in sera from 41 patients (22% of studied patients). IgE inhibition analysis revealed that IgE reactivity to the booklouse allergen in the sera from one third of booklouse-sensitized patients was not inhibited by preincubation with extracts from any other environmental insects in this study. Immunoblotting identified a 26-kD protein from booklouse extract as the allergenic protein contributing to specific sensitization to booklice. The amino acid sequence of peptide fragments of this protein showed no homology to those of previously described allergenic proteins, indicating that this protein is a new allergen.Conclusions:Sensitization to booklice was relatively common and specific sensitization to this insect not related to insect panallergy was indicated in this population.