Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing
Selective Detection and Characterization of Small Cysteine-Containing Peptides with Cluster-Modified Nanopore Sensing
复制标题
使用簇修饰纳米孔传感选择性检测和表征含半胱氨酸的小肽
DOI：
10.1021/acsnano.2c07842
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发表时间：
2022
期刊：
影响因子：
17.1
通讯作者：
Reiner, Joseph E.
中科院分区：
文献类型：
--
作者：
Ghimire, Madhav L.;Cox, Bobby D.;Winn, Cole A.;Rockett, Thomas W.;Schifano, Nicholas P.;Slagle, Hannah M.;Gonzalez, Frank;Bertino, Massimo F.;Caputo, Gregory A.;Reiner, Joseph E.
最近证明，可以使用连续脉冲纳米孔传感来监测配体诱导的单个硫醇盐封端的金纳米团簇的结构波动。波动的大小与封端配体的大小成比例，并且后来显示可以在该纳米孔设置中观察到配体交换。我们扩大这些结果，探索不同类型的电流波动与肽配体连接硫普罗宁帽金纳米簇。我们在这里表明，波动可用于识别附着肽通过肽诱导的电流跳跃或高频电流波动的发病的幅度。重要的是，肽连接过程要求肽含有半胱氨酸残基。这表明基于纳米孔的肽与硫醇盐封端的簇的连接的监测可以提供用于选择性检测含半胱氨酸的肽的手段。最后，我们展示了基于集群的协议与各种肽的混合物，表明可以识别一个以上的混合物中的含半胱氨酸的肽。
It was recently demonstrated that one can monitor ligand-induced structure fluctuations of individual thiolate-capped gold nanoclusters using resistive-pulse nanopore sensing. The magnitude of the fluctuations scales with the size of the capping ligand, and it was later shown one can observe ligand exchange in this nanopore setup. We expand on these results by exploring the different types of current fluctuations associated with peptide ligands attaching to tiopronin-capped gold nanoclusters. We show here that the fluctuations can be used to identify the attaching peptide through either the magnitude of the peptide-induced current jumps or the onset of high-frequency current fluctuations. Importantly, the peptide attachment process requires that the peptide contains a cysteine residue. This suggests that nanopore-based monitoring of peptide attachments with thiolate-capped clusters could provide a means for selective detection of cysteine-containing peptides. Finally, we demonstrate the cluster-based protocol with various peptide mixtures to show that one can identify more than one cysteine-containing peptide in a mixture.