Gold nanoparticle-based colorimetric methods to determine protein contents in artificial urine using membrane micro-concentrators and mobile phone camera
Gold nanoparticle-based colorimetric methods to determine protein contents in artificial urine using membrane micro-concentrators and mobile phone camera
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DOI：
10.1016/j.snb.2016.07.158
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发表时间：
2017-02-01
影响因子：
8.4
通讯作者：
Wang, Shau-Chun
中科院分区：
文献类型：
--
作者：
Lai, Teh-Sheng;Chang, Ting-Chou;Wang, Shau-Chun
在本文中，我们使用离子交换膜盘开发小型化浓集装置，该装置采用金纳米颗粒（GNP）比色法测定人工尿液样品中的人血清白蛋白含量。当在阳离子交换基板上施加外场时，当离子在阴极侧富集时，阳极侧出现离子耗尽，以满足质量守恒和电中性约束。特别是当离子交换基质为圆形时，穿过膜盘的场线在出口极处聚焦，以进一步增强浓差极化，从而产生超浓缩点。使用包含 Nafion 阳离子交换膜盘的小型化浓度芯片进行测定白蛋白的 GNP 比色测定。在碱性条件下，当GNP溶液中加入白蛋白并使用膜盘进行浓缩时，阴离子白蛋白可以防止浓缩的GNP聚集，并且即使在高盐溶液中，浓缩的GNP仍保持红色。另一方面，如果没有吸收白蛋白的有效保护，GNP 就会聚集并将颜色变为蓝色。使用智能手机摄像头获取非聚集纳米颗粒的浓度图像，发现红色强度与白蛋白浓度的对数值之间存在线性相关性，从而量化白蛋白量。该比色测定法经过验证，可测定含有肌酐和尿液的人造尿基质中具有临床意义的范围（0.1-20 μM）的主要尿蛋白、人血清白蛋白样品。这些结果证明了膜基微聚光装置与手机摄像头结合使用的可行性。 (C) 2016 Elsevier B.V. 保留所有权利。
In this paper, we use an ion-exchange membrane disk to develop miniaturized concentration device, which employs gold nanoparticle (GNP) colorimetric assay to determine human serum albumin content in artificial urine samples.When an external field is applied across cation exchange substrate, the ion depletion appears on the anodic side when the ions are enriched on the cathodic side to fulfill mass conservation and electrical neutrality constrains. In particular when the ion exchange substrates are of round shape, the field lines passing through the membrane disk are focused at the exit pole to further enhance concentration polarization to result in a superconcentraiton spot.GNP colorimetric assays to determine albumin are carried out using the miniaturized concentration chip containing a Nafion cation-exchange membrane disk. Under alkaline condition when GNP solution is spiked with albumins to concentrate using the membrane disk, anionic albumins can prevent the concentrated GNP's from aggregation and the concentrated GNP's remain in red color even in highly salty solutions. On the other hand without efficient protections by absorbed albumins, GNP's aggregate and change color to blue. Using smart phone camera to acquire the concentration images of non-aggregated nanoparticles, one linear correlation is found between the red color intensity and the logarithm values of albumin concentration to quantify albumin amounts. This colorimetric assay is validated to determine the samples of the main urine protein, human serum albumin at the range of clinical significance (0.1-20 mu M) in artificial urine matrix containing creatinine and urine. These results prove the feasibility of using the membrane-based micro-concentration device with mobile phone camera. (C) 2016 Elsevier B.V. All rights reserved.