GLYCINE METABOLISM BY RAT-LIVER MITOCHONDRIA .7. ISOLATION AND SOME PROPERTIES OF PROTEIN-BOUND INTERMEDIATE OF REVERSIBLE GLYCINE CLEAVAGE REACTION
GLYCINE METABOLISM BY RAT-LIVER MITOCHONDRIA .7. ISOLATION AND SOME PROPERTIES OF PROTEIN-BOUND INTERMEDIATE OF REVERSIBLE GLYCINE CLEAVAGE REACTION
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DOI：
10.1016/0003-9861(74)90075-7
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发表时间：
1974-01-01
影响因子：
3.9
通讯作者：
KIKUCHI, G
中科院分区：
文献类型：
--
作者：
MOTOKAWA, Y;KIKUCHI, G
从大鼠肝线粒体中分离出一种酶系统，该酶系统催化甘氨酸降解为一个碳单位、氨和二氧化碳，并从这三种物质合成甘氨酸。甘氨酸可逆切割系统由四种蛋白质组成，分别命名为P-、H-、L-和T-蛋白。描述了一种纯化P蛋白和T蛋白的方法，P蛋白催化甘氨酸的脱羧或在H蛋白存在下的逆反应，T蛋白参与一个碳单元和氨的形成或逆反应。所描述的程序导致分离出几乎同质形式的T蛋白，但P蛋白仍然是异质的。通过分子筛色谱法估计T蛋白的分子量为33，000。本文还介绍了合成反应、裂解反应和甘氨酸羧基与碳酸氢根交换反应的性质。
An enzyme system which catalyzes the degradation of glycine to one carbon unit, ammonia, and carbon dioxide and the synthesis of glycine from these three substances has been isolated from rat liver mitochondria. The reversible glycine cleavage system is composed of four protein components named as P-, H-, L-, and T-protein, respectively. A procedure is described for the purification of P-protein which catalyzes the decarboxylation of glycine or its reverse reaction in the presence of H-protein, and for T-protein which participates in the formation of one carbon unit and ammonia or the reverse reaction. The procedure described leads to the isolation of a nearly homogeneous form of T-protein but P-protein still is heterogeneous. The molecular weight of T-protein, estimated by molecular sieve chromatography, is 33,000. Properties of the synthesis and cleavage reactions and the exchange of carboxyl group of glycine with bicarbonate are also presented.