Growth Characteristics of Plant Hairy Roots and Their Cultures in Bioreactors
Growth Characteristics of Plant Hairy Roots and Their Cultures in Bioreactors
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植物毛状根的生长特性及其在生物反应器中的培养
DOI：
10.1252/jcej.22.84
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
C. Matsui
中科院分区：
文献类型：
--
作者：
M. Taya;A. Yoyama;O. Kondo;Takeshi Kobayashi;C. Matsui
由土壤细菌发根农杆菌转化的来自辣根、胡萝卜等的植物毛状根在以蔗糖作为碳源的不含植物激素的Murashige-Skoog培养基中表现出活跃的生长。网状聚氨酯泡沫是毛状根细胞固定化的理想载体。在固定化辣根细胞的气升式生物反应器中培养31 d，可获得11 kg干细胞·m-3（110 kg鲜细胞·m-3）的毛状根。毛状根培养物的干细胞生长量与培养基电导率下降呈线性关系。因此，它是可能的，以监测毛状根培养过程中的细胞质量浓度在线测量的电导率在生物反应器系统。
Plant hairy roots from horseradish, carrots, etc. transformed by the soil bacterium Agrobacterium rhizogenes showed active growth in the phytohormone-free Murashige–Skoog medium with sucrose as a carbon source. Reticulate polyurethane foam was an appropriate support for the immobilization of hairy root cells. The hairy roots of 11 kg-dry cells·m–3 (110 kg-fresh cells·m–3) were obtained in a 31-d culture in an air-lift column bioreactor with immobilized horseradish cells. A linear relationship was observed between the dry cell mass grown and medium conductivity decrease for hairy root cultures. Thus, it was possible to monitor cell mass concentration during the hairy root culture by on-line measurement of conductivity in the bioreactor system.