Sensitive assays for urinary retinol-binding protein and β-2-glycoprotein-1 based on commercially available standards
Sensitive assays for urinary retinol-binding protein and β-2-glycoprotein-1 based on commercially available standards
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DOI：
10.1177/000456329803500116
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发表时间：
1998-01-01
影响因子：
2.2
通讯作者：
Norden, AGW
中科院分区：
文献类型：
--
作者：
Lapsley, M;Akers, K;Norden, AGW
尿视黄醇结合蛋白 (RBP) 和 β-2-糖蛋白-1 (β 2G1) 的测量提供了近端肾小管功能障碍的敏感指标。 beta 2G1 在尿液中非常稳定，但 RBP 的稳定性要差得多，已得到更广泛的研究。我们设计了两种类似的免疫测定法，可以从一种尿液稀释液中测量两种蛋白质。这些检测均使用广泛使用的商业标准，并允许在大部分参考范围内进行准确测量。我们测量了 70 名健康成人和 80 名 3 至 16 岁健康儿童尿液中的两种蛋白质，并将结果表示为与肌酐浓度的比率。使用第 98 个百分位作为参考上限，成人排泄高达 17 μg/mmol RBP（中位数 8）和高达 30 μg/mmol β 2G1（中位数 15），性别之间没有差异。儿童与成人的排泄范围不同，RBP 高达 22 μg/mmol（中位数 10），β 2G1 高达 80 μg/mmol（中位数 24）。
Measurements of urinary retinol-binding protein (RBP) and beta-2-glycoprotein-1 (beta 2G1) provide sensitive indicators of proximal renal tubular malfunction. beta 2G1 is very stable in urine but RBP, which is much less stable, has been more extensively studied. We have designed two similar immunoassays allowing measurement of both proteins from one urine dilution. The assays both use widely available commercial standards and permit accurate measurement throughout most of the reference range. We measured both proteins in the urine of 70 healthy adults and 80 healthy children aged between three and 16 years, expressing the results as a ratio to creatinine concentration. Using the 98th centile as the upper reference limit, adults excreted up to 17 mu g/mmol RBP (median 8) and up to 30 mu g/mmol beta 2G1 (median 15) with no difference between the sexes. Children had different ranges to adults with excretion of up to 22 mu g/mmol RBP (median 10) and up to 80 mu g/mmol beta 2G1 (median 24).