Tn5030: a conjugative transposon conferring clindamycin resistance in Bacteroides species.
Tn5030: a conjugative transposon conferring clindamycin resistance in Bacteroides species.
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Tn5030：一种接合转座子，赋予拟杆菌属物种克林霉素抗性。
DOI：
10.1093/clinids/12.supplement_2.s235
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Macrina,FL
中科院分区：
文献类型：
--
作者：
Halula,M;Macrina,FL
在革兰氏阳性菌中，已经描述了几个在质粒DNA不存在的情况下抗生素抗性决定簇的接合转移的例子。以前的研究表明，克林霉素耐药性在革兰氏阴性厌氧菌种之间的转移可能通过类似的机制发生。我们在这里报告了Tn 5030的特征，它是一种45-kb ± 5-kb的染色体、移动的遗传元件，缺乏通常与革兰氏阴性转座子相关的可检测末端重复序列。Tn 5030转移克林霉素耐药（ermFU）和序列同源totetF在可检测的质粒DNA的情况下。该元件代表了革兰氏阴性生物之间遗传交换的新机制。
Several examples of conjugal transfer of antibiotic resistance determinants in the absence of plasmid DNA have been described in gram-positive species. Previous work had suggested that transfer of clindamycin resistance among the gram-negative anaerobicBacteroidesspecies might occur by a similar mechanism. Wereport here the characterization of Tn5030, a 45-kb ± 5-kb chromosomal, mobile genetic element devoid of the detectable terminal repeats typically associated with gram-negative transposons. Tn5030transfers clindamycin resistance (ermFU) and sequences homologous totetF in the absence of detectable plasmid DNA. This element represents a novel mechanism of genetic exchange among gram-negative organisms.