A MEMBRANE GUANYLATE-CYCLASE DROSOPHILA HOMOLOG GENE EXHIBITS MATERNAL AND ZYGOTIC EXPRESSION
A MEMBRANE GUANYLATE-CYCLASE DROSOPHILA HOMOLOG GENE EXHIBITS MATERNAL AND ZYGOTIC EXPRESSION
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DOI：
10.1006/dbio.1993.1255
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发表时间：
1993-10-01
影响因子：
2.7
通讯作者：
MALVA, C
中科院分区：
文献类型：
--
作者：
GIGLIOTTI, S;CAVALIERE, V;MALVA, C
鸟苷酸环化酶基因的假定膜形式在黑腹果蝇第二染色体的32区被鉴定。果蝇蛋白具有单一疏水序列，该序列将蛋白分为推定的胞外区和胞质催化区，其中包含酪氨酸激酶和环化酶结构域，与其他鸟苷酸和腺苷酸环化酶表现出不同程度的保守性。该基因显示出非常中断的组织，具有被小内含子分隔的小外显子和低水平的表达。转录本已定位于生殖细胞卵子发生期间以及随后卵室发育的第 10-14 阶段。推定的母体转录本可以在非常早期的胚胎中看到，并在后来作为合子基因表达的产物重新出现。
A putative membrane form of guanylate cyclase gene was identified in region 32 of the second chromosome ofDrosophila melanogaster. TheDrosophilaprotein has a single hydrophobic sequence that divides the protein into a putative extracellular region and a cytoplasmic catalytic region which contains tyrosine kinase and cyclase domains showing varying degrees of conservation with other guanylate and adenylate cyclases. The gene shows a very interrupted organization with small exons separated by small introns and a low level of expression. Transcripts have been localizedin situduring oogenesis in the germarium and later in stages 10-14 of egg chamber development. The putative maternal transcript can be seen in very early embryos and reappears later as a product of zygotic gene expression.