Time-course responses of circulating microRNAs to three resistance training protocols in healthy young men.
Time-course responses of circulating microRNAs to three resistance training protocols in healthy young men.
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DOI：
10.1038/s41598-017-02294-y
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发表时间：
2017-05-19
影响因子：
4.6
通讯作者：
Ma J
中科院分区：
文献类型：
--
作者：
Cui S;Sun B;Yin X;Guo X;Chao D;Zhang C;Zhang CY;Chen X;Ma J
人类血浆中的循环microRNAs(c-miRNAs)已被描述为一种潜在的运动标志物。本研究探讨了三种急性抗阻训练(RT)方案对青年男性c-miRNAs谱时程变化的影响。将受试者(n = 45)随机分为3组：肌肉力量耐力(SE)组、肌肉肥大(MH)组和最大力量(MS)组。分别于运动前、运动后即刻、1 h和24 h采集静脉血，测定c-miRNA、合成和分解激素、炎性细胞因子和肌肉损伤标志物。结果表明，2个c-miRNAs(miR-208b和miR-532)、6个c-miRNAs(miR-133a、miR-133b、miR-206、miR-181a、miR-21和miR-221)和2个c-miRNAs(miR-133a和miR-133b)的水平分别随着SE、MH和MS协议(p <； 0.05)的变化而显著变化。C-miRNAs反应的性质和动态过程可能受到RT方式和强度的影响。MiR-532与胰岛素样生长因子-1呈负相关，与白细胞介素10呈正相关，miR-133a与皮质醇呈负相关，与睾酮/皮质醇呈正相关。这些发现表明，这些c-miRNAs可以作为监测RT反应的标志物。
Circulating microRNAs (c-miRNAs) in human plasma have been described as a potential marker of exercise. The present study investigated the effects of three acute resistance training (RT) protocols on the time-course changes of the c-miRNAs profiles in young males. The subjects (n = 45) were randomly divided into three groups: muscular strength endurance (SE), muscular hypertrophy (MH) and maximum strength (MS). Venous blood samples were obtained before exercise and immediately, 1 h and 24 h after each RT protocol to assess the following biological parameters: c-miRNAs, anabolic and catabolic hormones, inflammatory cytokines and muscle damage markers. The results revealed that the levels of two c-miRNAs (miR-208b and miR-532), six c-miRNAs (miR-133a, miR-133b, miR-206, miR-181a, miR-21 and miR-221) and two c-miRNAs (miR-133a and miR-133b) changed significantly in response to the SE, MH and MS protocols (p < 0.05), respectively. The nature and dynamic processes of the c-miRNAs response were likely influenced by the RT modality and intensity. Moreover, miR-532 was negatively correlated with insulin-like growth factor-1 and positively correlated with interleukin-10, whereas miR-133a was negatively correlated with cortisol and positively correlated with testosterone/cortisol. These findings suggest that these c-miRNAs may serve as markers for monitoring the RT responses.