Changes in intrarenal oxygenation as evaluated by BOLD MRI in a rat kidney model for radiocontrast nephropathy
Changes in intrarenal oxygenation as evaluated by BOLD MRI in a rat kidney model for radiocontrast nephropathy
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DOI：
10.1002/jmri.1103
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发表时间：
2001-05-01
影响因子：
4.4
通讯作者：
Epstein, FH
中科院分区：
文献类型：
--
作者：
Prasad, PV;Priatna, A;Epstein, FH
目前对造影剂肾病的发病机制知之甚少。在动物模型中，抑制一氧化氮和前列腺素的合成似乎使大鼠在给予放射造影剂后容易发生严重的肾脏损伤。在这里，我们调查了是否可以通过血液氧合水平依赖(BOLD)MRI来评估放射造影剂的应用，以及药物抑制一氧化氮和前列腺素合成后肾髓质氧合的变化。对19只麻醉大鼠(肌动蛋白100 mg/kg)进行了研究。注射L硝基精氨酸甲酯(N-硝基L精氨酸甲酯，10 mg/kg)、吲哚美辛(10 mg/kg)和放射造影剂(60%硫代巴比妥钠，6mL/kg)后进行BOLD MRI测量。在每一次药物干预后，延髓R-2*的显著连续变化，可能反映了延髓Po(2)的下降。这些由无创性磁共振成像获得的结果与先前直接记录的大鼠肾髓质在L-NAME、吲哚美辛和硫代氨基甲酸酯后的Po(2)和多普勒血流一致。通过抑制前列腺素和一氧化氮的合成以及静脉注射放射造影剂，可以减少大鼠的髓质氧合。BOLD AM可以无创性地评估似乎易导致急性肾功能衰竭的大鼠骨髓氧合的变化。(C)2001年Wiley-Liss，Inc.
The pathogenesis of radiocontrast nephropathy is poorly understood. In an animal model, inhibition of the synthesis of nitric oxide and prostaglandins appears to predispose rats to severe renal injury following the administration of radiocontrast. Here we have investigated whether administration of radiocontrast, as well as changes in renal medullary oxygenation following pharmacologic inhibition of nitric oxide and prostaglandin synthesis, might be evaluated by blood oxygenation level-dependent (BOLD) MRI. Nineteen anesthetized (Inactin 100 mg/kg) rats were studied. BOLD MRI measurements were performed following administration of L-NAME (N-nitro-L-arginine methyl ester, 10 mg/kg), Indomethacin (10 mg/kg), and a radiocontrast agent (sodium iothalamate 60%, 6 mL/kg). Marked sequential changes in medullary R-2*, presumably reflecting decline in medullary pO(2), were noted after each of the pharmacological interventions employed. These results, obtained by noninvasive MRI, are consistent with prior direct recordings Of pO(2) and doppler flow in the rat renal medulla after administration of L-NAME, Indomethacin and iothalamate. Medullary oxygenation in rats was reduced by inhibition of the synthesis of prostaglandins and nitric oxide, as well as by intravenous injection of radiocontrast agents. BOLD AM can noninvasively evaluate changes in medullary oxygenation in rats that appear to predispose acute renal failure. (C) 2001 Wiley-Liss, Inc.