Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7
Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7
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DOI：
10.1038/emboj.2013.15
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发表时间：
2013-03-06
期刊：
影响因子：
11.4
通讯作者：
Zipfel, Cyril
中科院分区：
文献类型：
--
作者：
Nicaise, Valerie;Joe, Anna;Zipfel, Cyril
病原体靶向宿主免疫的重要成分以引起疾病。假单胞菌III型分泌的效应HopU 1是一种单ADP核糖基转移酶，对拟南芥具有完全的毒力。HopU 1靶向几种RNA结合蛋白，包括GRP 7，其在免疫中的作用尚不清楚。在这里，我们表明，GRP 7与翻译组件，以及与模式识别受体FLS 2和EFR。此外，GRP 7通过其RNA识别基序在体内特异性结合FLS 2和EFR转录物。HopU 1不影响GRP 7、FLS 2和翻译组分之间的蛋白质-蛋白质结合。相反，HopU 1阻断了GRP 7和FLS 2与EFR转录本之间的体内相互作用。这种抑制作用与假单胞菌感染后FLS 2蛋白水平的降低相关，这是HopU 1依赖性的。我们的研究结果揭示了一种新的毒力策略使用的微生物效应干扰宿主免疫。The EMBO Journal（2013）32，701-712. doi：10.1038/2013.15; 2013年2月8日在线发布
Pathogens target important components of host immunity to cause disease. The Pseudomonas syringae type III-secreted effector HopU1 is a mono-ADP-ribosyltransferase required for full virulence on Arabidopsis thaliana. HopU1 targets several RNA-binding proteins including GRP7, whose role in immunity is still unclear. Here, we show that GRP7 associates with translational components, as well as with the pattern recognition receptors FLS2 and EFR. Moreover, GRP7 binds specifically FLS2 and EFR transcripts in vivo through its RNA recognition motif. HopU1 does not affect the protein-protein associations between GRP7, FLS2 and translational components. Instead, HopU1 blocks the interaction between GRP7 and FLS2 and EFR transcripts in vivo. This inhibition correlates with reduced FLS2 protein levels upon Pseudomonas infection in a HopU1-dependent manner. Our results reveal a novel virulence strategy used by a microbial effector to interfere with host immunity. The EMBO Journal (2013) 32, 701-712. doi:10.1038/emboj.2013.15; Published online 8 February 2013