Gel microstructure regulates proliferation and differentiation of MC3T3-E1 cells encapsulated in alginate beads.
Gel microstructure regulates proliferation and differentiation of MC3T3-E1 cells encapsulated in alginate beads.
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DOI：
10.1016/j.actbio.2012.01.012
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发表时间：
2012-05
影响因子：
9.7
通讯作者：
Guelcher, Scott A.
中科院分区：
文献类型：
--
作者：
Lee, Baek-Hee;Li, Bing;Guelcher, Scott A.
为了将细胞移植到受损组织中，必须将活细胞在合适的载体中递送到缺损部位。然而，载体中的缺氧和营养有限的环境可诱导大量细胞死亡。本研究的目的是通过控制海藻酸钠水凝胶的微观结构来提高骨祖细胞的活力并调节其行为。通过使用α-MEM作为海藻酸钠盐和CaCl 2溶液的溶剂，改善了藻酸盐珠中的细胞存活率，与水或缓冲液相比，其为细胞提供了额外的营养。假设水凝胶的网格尺寸和剪切模量调节骨祖细胞的增殖和分化。MC 3 T3-E1细胞表现出增强成骨细胞分化时，封装在高密度藻酸盐具有更小的网眼尺寸和更刚性的机械性能，证实了碱性磷酸酶活性和骨钙素分泌增加。然而，MC 3 T3-E1细胞包封在低密度藻酸盐珠具有较大的网眼尺寸和更顺应性的机械性能表现出增加的增殖。这些结果表明，海藻酸钠水凝胶的微观结构可以调节骨祖细胞的行为，从而表明，调整凝胶的性质可能是一个有用的方法，以促进新骨形成。
For cell transplantation into damaged tissues, viable cells must be delivered to the defect site in a suitable carrier. However, the hypoxic and nutrient-limited environment in the carrier can induce massive cell death. The aims of this study were to increase the viability and regulate the behavior of osteoprogenitor cells encapsulated in alginate hydrogels through control of the gel microstructure. Cell survivability in alginate beads was improved through the use of α-MEM as the solvent for alginic acid sodium salt and CaCl2 solutions, which supplied additional nutrients for the cells compared to water or buffer. The mesh size and shear modulus of the hydrogel were hypothesized to regulate proliferation and differentiation of osteoprogenitor cells. MC3T3-E1 cells demonstrated enhanced osteoblast differentiation when encapsulated in high-density alginate with smaller mesh size and more rigid mechanical properties, as confirmed by increased alkaline phosphatase activity and osteocalcin secretion. However, MC3T3-E1 cells encapsulated in low-density alginate beads with a larger mesh size and more compliant mechanical properties exhibited increased proliferation. These results demonstrate that the microstructure of alginate hydrogels can regulate the behavior of osteoprogenitor cells, thus suggesting that the tuning the properties of the gel may be a useful approach for enhancing new bone formation.
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影响因子：
9.7
作者：
Fonseca, Keila B.;Bidarra, Silvia J.;Barrias, Cristina C.
通讯作者：
Barrias, Cristina C.
影响因子：
37.8
作者：
Hofmann, M;Wollert, KC;Drexler, H
通讯作者：
Drexler, H
影响因子：
41.2
作者：
Kong, HJ;Liu, JD;Mooney, DJ
通讯作者：
Mooney, DJ
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM
影响因子：
14
作者：
Comisar, Wendy A.;Kazmers, Nikolas H.;Linderman, Jennifer J.
通讯作者：
Linderman, Jennifer J.