The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)
The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)
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DOI：
10.1039/c7gc01696d
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发表时间：
2017-10-07
期刊：
影响因子：
9.8
通讯作者：
Carnell, A. J.
中科院分区：
文献类型：
--
作者：
McKenna, S. M.;Mines, P.;Carnell, A. J.
通过控制反应条件，以半乳糖氧化酶M3-5、周质醛氧化酶（PaoABC）、过氧化氢酶和辣根过氧化物酶为催化剂，采用一锅法连续氧化羟甲基糠醛（HMF）制备呋喃二甲酸（FDCA）。此外，PaoABC被成功地包埋在SiO2水凝胶和回收14次，没有失去活性。该催化剂能够耐受高达200 mM DFF浓度，得到完全转化的FDCA，具有非常有希望的TOF和TON值。
By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M3-5, periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO2 hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values.