Influence of Oxidative and Nitrosative Stress on Accumulation of Diphosphate Intermediates of the Non-mevalonate Pathway of Isoprenoid Biosynthesis in Corynebacteria and Mycobacteria
Influence of Oxidative and Nitrosative Stress on Accumulation of Diphosphate Intermediates of the Non-mevalonate Pathway of Isoprenoid Biosynthesis in Corynebacteria and Mycobacteria
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DOI：
10.1134/s0006297912040074
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Kapreliants, A. S.
中科院分区：
文献类型：
--
作者：
Artsatbanov, V. Yu.;Vostroknutova, G. N.;Kapreliants, A. S.
人工生成氧超氧自由基在结核分枝杆菌积极生长的培养，Myc。然后在细菌细胞中积累大量的2- c -甲基- d - erythrit醇-2,4-cyclodiphosphate (MEcDP)， MEcDP是类异戊二烯生物合成（MEP）的非甲羟酸途径的中间体，很可能是由于氧自由基与活性中心的4Fe-4S基团相互作用和抑制酶(E)-4-氧-3-甲基-2-烯基二磷酸合成酶（IspG）。镉离子在叶绿体中抑制IspG酶(Rivasseau， C, Seemann， M., Boisson， A. M., Streb， P., Gout， E., Douce， R., Rohmer， M., and Bligny， R. (2009) Plant Cell Environ。， 32,82 -92)，添加到Myc。氧化应激诱导的MEcDP积累显著增加，而细胞中没有其他有机磷酸盐中间体的积累。以能够合成大量MEcDP而闻名的氨根棒状杆菌也被证明在活跃生长的培养基中，当在培养基中人工产生低浓度的NO时，可以积累这种独特的环二磷酸。本文讨论了类异戊二烯生物合成mep通路及其中心中间体MEcDP在细菌对亚硝化和氧化应激反应中的可能作用。
Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcDP) - an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (MEP) - most possibly due to the interaction of the oxygen radicals with the 4Fe-4S group in the active center and inhibition of the enzyme (E)-4-oxy-3-methylbut-2-enyl diphosphate synthase (IspG). Cadmium ions known to inhibit IspG enzyme in chloroplasts (Rivasseau, C., Seemann, M., Boisson, A. M., Streb, P., Gout, E., Douce, R., Rohmer, M., and Bligny, R. (2009) Plant Cell Environ., 32, 82-92), when added to culture of Myc. smegmatis, substantially increase accumulation of MEcDP induced by oxidative stress with no accumulation of other organic phosphate intermediates in the cell. Corynebacterium ammoniagenes, well-known for its ability to synthesize large amounts of MEcDP, was also shown to accumulate this unique cyclodiphosphate in actively growing culture when NO at low concentration is artificially generated in the medium. A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed.