SCAR/WAVE: A complex issue.
SCAR/WAVE: A complex issue.
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DOI：
10.4161/cib.27033
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发表时间：
2013-11-01
影响因子：
--
通讯作者：
Insall RH
中科院分区：
文献类型：
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作者：
Davidson AJ;Insall RH
SCAR/WAVE复合物驱动肌动蛋白聚合，肌动蛋白聚合是细胞前部突出的基础，从而驱动迁移。然而，目前尚不清楚如何调节SCAR/WAVE的活性以产生在细胞运动期间观察到的无限范围的细胞形状变化。SCAR/WAVE复合体的亚基的相对作用是什么？它们与哪些信号分子相互作用？在突起形成的过程中，复合物是如何整合所有这些信息，以控制肌动蛋白的时空聚合的？不幸的是，SCAR复合体成员的相互依赖性使得基因解剖变得困难。在我们最近的论文中，1我们描述了一个小片段阿比特龙的Dictyosteoblastoma SCAR复合物的稳定性。在这里，我们总结了主要的研究结果，并讨论了这种方法如何有助于揭示这个不可穿透的复杂的内部运作。
The SCAR/WAVE complex drives the actin polymerisation that underlies protrusion of the front of the cell and thus drives migration. However, it is not understood how the activity of SCAR/WAVE is regulated to generate the infinite range of cellular shape changes observed during cell motility. What are the relative roles of the subunits of the SCAR/WAVE complex? What signaling molecules do they interact with? And how does the complex integrate all this information in order to control the temporal and spatial polymerisation of actin during protrusion formation? Unfortunately, the interdependence of SCAR complex members has made genetic dissection hard. In our recent paper,1 we describe stabilization of the Dictyostelium SCAR complex by a small fragment of Abi. Here we summarize the main findings and discuss how this approach can help reveal the inner workings of this impenetrable complex.