Glucocerebroside transfer between phosphatidylcholine bilayers.
Glucocerebroside transfer between phosphatidylcholine bilayers.
复制标题
葡萄糖脑苷脂在磷脂酰胆碱双层之间转移。
DOI：
10.1021/bi00535a021
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Thompson,TE
中科院分区：
文献类型：
--
作者：
Correa-Freire,MC;Barenholz,Y;Thompson,TE
Maria C. Correa-Freire、Y. Barenholz** 和 TE Thompson* 摘要：我们通过使用芘和 3H 标记的葡萄糖脑苷脂掺入二肉豆蔻酰磷脂酰胆碱 (DMPC) 和二棕榈酰磷脂酰胆碱 (DPPC) 双层中，研究了葡萄糖脑苷脂在磷脂双层之间转移的动力学。芘标记的葡萄糖脑苷脂 (PyrCer) 分子能够在基态 PyrCer 和激发单体 (M) 之间形成激发复合物（准分子，E）。当含有已知量的 PyrCer（供体）的囊泡与未标记的囊泡（受体）一起孵育时，PyrCer 从供体到受体群体的转移反映在观察到的 E/M 强度比的降低。从这些研究中获得的结果表明，从供体 DMPC-PyrCer 转移的半衰期
Maria C. Correa-Freire, Y. Barenholz,** and TE Thompson* abstract: We have studied the kinetics of transfer of glu-cocerebroside between phospholipid bilayers by using pyrene and 3H-labeled glucocerebroside incorporated into di-myristoylphosphatidylcholine (DMPC) and dipalmitoyl-phosphatidylcholine (DPPC) bilayers. Pyrene-labeled glucocerebroside (PyrCer) molecules are able to form an excited complex (eximer, E) between a PyrCer in the ground state and an excited monomer(M). When vesicles containing a known amount of PyrCer (donors) are incubated with unla-beled vesicles (acceptors), transfer of PyrCer from donor to acceptor populations is reflected in a decrease of the observed E/M intensity ratio. The results obtained from these studies show that the half-time of transfer from donor DMPC-PyrCer