Septicine Inhibits the Production of Inflammatory Mediators in Lipopolysaccharide-Stimulated Murine Macrophages
Septicine Inhibits the Production of Inflammatory Mediators in Lipopolysaccharide-Stimulated Murine Macrophages
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败血碱抑制脂多糖刺激的小鼠巨噬细胞中炎症介质的产生
DOI：
10.5352/jls.2011.21.9.1310
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Jin
中科院分区：
文献类型：
--
作者：
Geun;Jin
在脂多糖（LPS）刺激的小鼠巨噬细胞RAW264.7细胞中，研究了存在于卵形蝗叶和茎中的天然生物碱产物septicine的抗炎活性。用septicine处理后，lps诱导的一氧化氮（NO）、炎症细胞因子、肿瘤坏死因子-α和白细胞介素-6的产生呈浓度依赖性。此外，果皮素抑制了诱导型NO合成酶的表达。上述结果提示，果仁的抗炎作用可能与抑制NO、iNOS及细胞因子的表达有关。
Anti-inflammatory activities of septicine, a natural alkaloid product present in the leaves and stems of Tylophora ovata, were evaluated in lipopolysaccharide (LPS)-stimulated murine macrophages, RAW264.7 cells. Treatment with septicine inhibited LPS-induced nitric oxide (NO), inflammatory cytokines, tumor necrosis factor-α and interleukin-6 production in a concentration-dependant manner. In addition, septicine suppressed the expression of inducible NO synthase. These results suggest that the anti-inflammatory activities of septicine might be attributed to the inhibition of NO, iNOS and cytokine expression.