Dual isotype expressing B cells [κ(+)/λ(+)] arise during the ontogeny of B cells in the bone marrow of normal nontransgenic mice
Dual isotype expressing B cells [κ(+)/λ(+)] arise during the ontogeny of B cells in the bone marrow of normal nontransgenic mice
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DOI：
10.1016/j.cellimm.2005.12.004
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发表时间：
2005-11-01
影响因子：
4.3
通讯作者：
Longo, DL
中科院分区：
文献类型：
--
作者：
Rezanka, LJ;Kenny, JJ;Longo, DL
克隆选择理论的核心是单个B细胞表达具有单一特异性的单一受体。以前，基于我们在抗磷酸胆碱转基因小鼠模型中的工作，我们认为B细胞通过在其细胞表面共表达一种以上的受体来逃避克隆缺失。我们认为，"受体稀释"是必要的，当：（i）表达的免疫球蛋白受体是免疫保护对病原体是必不可少的，（ii）这种保护性受体是自身反应性的，将被克隆删除，留下一个洞的B细胞库。在这里，我们证明了双同种型表达B细胞出现在正常个体发育过程中的B细胞在骨髓和人口的非转基因小鼠的脾脏和腹腔。此外，表达的免疫球蛋白轻链的单细胞分析表明，受体编辑可能在产生显著部分的表达双重同种型的B细胞中起作用。由爱思唯尔公司出版
Central to the clonal selection theory is the tenet that a single B cell expresses a single receptor with a single specificity. Previously, based on our work in anti-phosphocholine transgenic mouse models, we suggested that B cells escaped clonal deletion by coexpression of more than one receptor on their cell surface. We argued that '' receptor dilution '' was necessary when: (i) the expressed immunoglobulin receptor is essential for immune protection against pathogens and (ii) this protective receptor is autoreactive and would be clonally deleted, leaving a hole in the B cell repertoire. Here, we demonstrate that dual isotype expressing B cells arise during the normal ontogeny of B cells in the bone marrow and populate both the spleen and peritoneal cavity of nontransgenic mice. Furthermore, single cell analysis of the expressed immunoglobulin light chains suggests that receptor editing may play a role in the generation of a significant fraction of dual isotype expressing B cells. Published by Elsevier Inc.