Decolorization of Orange I under alkaline and anaerobic conditions by a newly isolated humus-reducing bacterium, Planococcus sp. MC01
Decolorization of Orange I under alkaline and anaerobic conditions by a newly isolated humus-reducing bacterium, Planococcus sp. MC01
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DOI：
10.1016/j.ibiod.2013.02.016
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发表时间：
2013-09
影响因子：
4.8
通讯作者：
Chen Ma;Shungui Zhou;Qin Lu;Guiqin Yang;Dingmei Wang;L. Zhuang;Fangbai Li;Famao Lei
中科院分区：
文献类型：
--
作者：
Chen Ma;Shungui Zhou;Qin Lu;Guiqin Yang;Dingmei Wang;L. Zhuang;Fangbai Li;Famao Lei
印染废水的pH值一般在9.0-13.0之间，但能处理印染废水的嗜碱细菌很少分离。在这里，我们报告了一个嗜碱和耐盐，腐殖质减少兼性厌氧菌，Planococcussp。MC 01（CGMCC 4771 = KCTC 33120），在碱性和厌氧条件下，能有效还原AQDS（蒽醌-2，6-二磺酸盐，腐殖质类似物），对橙子I脱色率>94.0%。橙子I的脱色过程符合准一级动力学，当醋酸盐、葡萄糖、蔗糖和乳酸盐作为电子给体时，速率常数（k）分别为0.12、0.17、0.14和0.12 h-1。当加入0.5 mmol l−1AQDS和2.0 mmol l−1γ-FeOOH作为电子穿梭时，脱色过程分别被刺激了44.4%和32.8%。此外，菌株MC 01在低初始浓度的橙子I（0.01-0.2 mmol l−1）下表现出高脱色活性，并且脱色的最佳葡萄糖浓度为10.0 mmol l−1。紫外-维斯光谱分析结果表明，脱色过程中偶氮双键发生断裂。据我们所知，这是第一个报告的嗜碱兼性厌氧菌能够脱色橙子I在碱性条件下。
Printing and dyeing wastewater (PDW) normally has a high pH of 9.0–13.0, but alkaliphilic bacteria capable of treating PDW have rarely been isolated. Here we report an alkaliphilic and halotolerant, humus-reducing facultative anaerobe,Planococcussp. MC01 (CGMCC 4771 = KCTC 33120), which can effectively reduce AQDS (anthraquinone-2, 6-disulphonate, humus analog) and decolorize Orange I (>94.0%) under alkaline and anaerobic conditions. The decolorization process of Orange I fits a pseudo-first-order kinetics well, and the rate constants (k) were 0.12, 0.17, 0.14, and 0.12 h−1when acetate, glucose, sucrose, and lactate, respectively, served as electron donor. When 0.5 mmol l−1AQDS and 2.0 mmol l−1γ-FeOOH were added as electron shuttles, the decolorization process was stimulated by 44.4% and 32.8%, respectively. Additionally, strain MC01 showed high decolorizing activity with low initial concentrations of Orange I (0.01–0.2 mmol l−1), and the optimal glucose concentration for decolorization was 10.0 mmol l−1. Results of UV/vis spectra suggested the cleavage of the double azo bond during decolorization. To the best of our knowledge, this is the first report of an alkaliphilic facultative anaerobe capable of decolorizing Orange I under alkaline conditions.