Antibody quantity versus quality after influenza vaccination
Antibody quantity versus quality after influenza vaccination
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DOI：
10.1016/j.vaccine.2009.06.090
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发表时间：
2009-10-23
期刊：
影响因子：
5.5
通讯作者：
Air, Gillian M.
中科院分区：
文献类型：
--
作者：
Feng, JingQi;Gulati, Upma;Air, Gillian M.
保护免受流感感染的相关因素尚未完全确定。我们对三组接种疫苗的受试者应用了ELISA策略，该策略将针对天然病毒表面抗原的抗体（可能中和）与针对内部和变性病毒蛋白的抗体（非中和）区分开来：（1）每年重复接种疫苗研究的参与者，（2）老年受试者和（3）与对照受试者相比的系统性红斑狼疮患者。接种后抗体增加与各组中既存抗体水平呈负相关;大多数受试者的初始抗体水平显著，接种后抗体量几乎没有增加，但其血清抗体的亲和力有增加的趋势。针对变性病毒蛋白的抗体随疫苗配方而变化;对于相同量的天然血凝素，最近的疫苗具有较少的总蛋白。我们提出了一个指数，包括抗体水平加上抗体亲合力的排名顺序，都对天然病毒，加上血凝抑制抗体滴度，作为一个有用的措施，对流感的免疫力。(C)2009爱思唯尔有限公司保留所有权利。
The correlates for protection against influenza infection are incompletely characterized. We have applied an ELISA strategy that distinguishes antibodies against native viral surface antigens (potentially neutralizing) from antibodies directed against internal and denatured viral proteins (not neutralizing) to three groups of vaccinated subjects: (1) participants in a study of repeated annual vaccination, (2) elderly subjects and (3) patients with Systemic Lupus Erythematosus compared to control subjects. Antibody increase after vaccination was inversely related to the level of pre-existing antibodies in all groups; most subjects had significant initial antibody levels and showed little increase in amount of antibody after vaccination, but the avidity of their serum antibodies tended to increase. Antibodies against denatured virus proteins varied with vaccine formulation; vaccines that are more recent have less total protein for the same amount of native hemagglutinin. We propose an index consisting of rank order of antibody level plus antibody avidity, both measured against native virus, plus hemagglutination-inhibition antibody titer, as a useful measure of immunity against influenza. (C) 2009 Elsevier Ltd. All rights reserved.