ISOLATION, CHARACTERIZATION, AND LOCALIZATION OF CARDIAC COLLAGEN TYPE-VI - ASSOCIATIONS WITH OTHER EXTRACELLULAR-MATRIX COMPONENTS
ISOLATION, CHARACTERIZATION, AND LOCALIZATION OF CARDIAC COLLAGEN TYPE-VI - ASSOCIATIONS WITH OTHER EXTRACELLULAR-MATRIX COMPONENTS
复制标题
DOI：
10.1161/01.res.70.5.1006
复制
发表时间：
1992-05-01
影响因子：
20.1
通讯作者：
JIMENEZ, SA
中科院分区：
文献类型：
--
作者：
BASHEY, RI;MARTINEZHERNANDEZ, A;JIMENEZ, SA
我们已经从鼠、犬和非人灵长类动物心脏中分离出VI型胶原蛋白并进行了表征。在所研究的三个物种中，I型胶原蛋白是心脏内膜的主要胶原成分（占总胶原蛋白的80%），而VI型胶原蛋白约占总胶原蛋白的5%。为了确定VI型胶原蛋白的确切分布及其与心脏细胞外基质的其他组分的可能相互作用，使用单特异性抗体通过免疫组织化学在大鼠心肌中定位I、III、IV和VI型胶原蛋白、层粘连蛋白和纤连蛋白。在大鼠心肌中，VI型胶原蛋白普遍存在于肌动脉的中膜和外膜、精细的结缔组织隔膜、毛细血管周围区域以及靠近心肌细胞的精致肌内膜中。与I型、III型和IV型胶原、层粘连蛋白和纤连蛋白的免疫组织化学定位相比，心脏细胞外基质的连续性和层次组织变得明显。基质形成从心包延伸到内膜的连续网络。此外，系统中存在树状结构层级，使得I型胶原蛋白在较宽的隔膜中更普遍，III型胶原蛋白在中等大小的分支中更明显，纤连蛋白和VI型胶原蛋白在网络的末端（细胞周）方面占主导地位。在这种细胞周围的位置，纤连蛋白和VI型胶原蛋白，通过特异性相互作用，可以作为锚组件连接心肌细胞基底膜不仅细胞外基质，而且心脏间质细胞。心脏细胞外基质的这种连续性、组织性和偶联性似乎非常适合于整合和分布由心肌的连续收缩和舒张产生的物理应力。
We have isolated and characterized collagen type VI from murine, canine, and nonhuman primate hearts. In the three species studied, collagen type I was the major collagenous component of the cardiac interstitium (80% of total collagen), whereas collagen type VI represented approximately 5% of total collagen. To define the exact distribution of collagen type VI and its possible interactions with other components of the cardiac extracellular matrix, collagen types I, III, IV, and VI, laminin, and fibronectin were localized in the rat myocardium by immunohistochemistry, using monospecific antibodies. In the rat myocardium, collagen type VI was prevalent in the media and adventitia of muscular arteries, in fine connective tissue septa, in the area surrounding capillaries, and in the delicate endomysium in proximity to myocardial cells. When compared with the immunohistochemical localization of collagen types I, III, and IV, laminin, and fibronectin, the continuity and hierarchical organization of the cardiac extracellular matrix became apparent. The matrix forms a continuous network extending from the pericardium to the endocardium. Furthermore, there is an arborescent hierarchy in the system such that collagen type I is more prevalent in the wider septa, collagen type III being more obvious in medium-sized branches, and fibronectin and collagen type VI prevailing in the terminal (pericellular) aspects of the network. In this pericellular location, fibronectin and collagen type VI, by means of specific interactions, may act as anchor components linking the myocardial cell basement membranes not only to the extracellular matrix but also to the cardiac interstitial cells. This continuity, organization, and coupling of the cardiac extracellular matrix appears well suited to integrate and distribute the physical stress generated by the continuous contraction and relaxation of the myocardium.