A MONOCLONAL-ANTIBODY THAT RECOGNIZES SIALYL-LEA OLIGOSACCHARIDE, BUT IS DISTINCT FROM NS-19-9 AS TO EPITOPE RECOGNITION
A MONOCLONAL-ANTIBODY THAT RECOGNIZES SIALYL-LEA OLIGOSACCHARIDE, BUT IS DISTINCT FROM NS-19-9 AS TO EPITOPE RECOGNITION
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DOI：
10.1093/oxfordjournals.jbchem.a122555
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发表时间：
1988-11-01
影响因子：
2.7
通讯作者：
YAMASHINA, I
中科院分区：
文献类型：
--
作者：
KITAGAWA, H;NAKADA, H;YAMASHINA, I
使用人结肠癌细胞系SW 1116作为免疫原产生鼠单克隆抗体，命名为MSW 113。MSW 113显示主要针对具有唾液酸-Lea抗原的粘蛋白型寡糖。MSW 113对唾液酸-Lea的反应性比NS 19-9更强，据信NS 19-9是针对相同的决定簇基团产生的，MSW 113结合唾液酸-Lea-醇、LS-四糖α，和二唾液酸乳糖-N-四糖，与NS 19-9相比具有更高的亲和力。这两种抗体可以清楚地区分，因为MSW 113结合唾液酸但不结合岩藻糖，而NS 19-9结合岩藻糖但不结合唾液酸。因此，MSW 113更多地指向含唾液酸的末端结构，而NS 19-9指向含岩藻糖的内部结构。MSW 113被发现可用于检测患者血流中的抗原，尤其是胰腺癌患者。即使NS 19-9阴性患者血清对MSW 113也呈阳性。
A murine monoclonal antibody, designated as MSW 113, was generated using a human colonic cancer cell line, SW 1116, as the immunogen. MSW 113 was shown to be directed mainly to mucin-type oligosaccharide with sialyl-Lea antigens. The reactivity of MSW 113 to sialyl-Lea was stronger than that of NS 19-9, which is believed to be raised against the same determinant group, MSW 113 binds to sialyl-Lea-ol, LS-tetrasaccharide .alpha., and disialyllacto-N-tetraose with higher affinities, compared to NS 19-9. These two antibodies could clearly be distinguished in that MSW 113 bound to sialic acid but not to fucose, whereas NS 19-9 bound to fucose but not to sialic acid. Thus, MSW 113 is directed more toward sialic acid-containing terminal structures while NS 19-9 is directed toward fucose-containing internal structures. MSW 113 was found to be useful for detecting antigens in the bloodstream of patients, especially those with pancreas cancer. Even NS 19-9 negative patient sera were positive for MSW 113.