A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles
A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles
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DOI：
10.1038/nnano.2009.195
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发表时间：
2009-09-01
影响因子：
38.3
通讯作者：
Nienhaus, G. Ulrich
中科院分区：
文献类型：
--
作者：
Roecker, Carlheinz;Poetzl, Matthias;Nienhaus, G. Ulrich
现在已知，当纳米颗粒暴露于生物液体时，会被蛋白质和其他生物分子包裹，形成“蛋白质冠”(1)。最近的系统研究已经确定了可以构成这种冠状的各种蛋白质，但这些纳米颗粒-蛋白质相互作用仍然知之甚少，并且表征它们的定量研究数量很少。在这里，我们利用荧光相关光谱法定量分析了人血清白蛋白在小（直径 10-20 nm）聚合物涂覆的 FePt 和 CdSe/ZnS 纳米粒子上的吸附。蛋白冠形成厚度为3.3 nm的单层。蛋白质以微摩尔亲和力与带负电的纳米颗粒结合，时间分辨荧光猝灭实验表明它们在颗粒上停留了大约 100 秒。这些新发现加深了我们对蛋白质电晕的定量理解，这对于纳米级物体在生物体中的安全应用至关重要。
It is now known that nanoparticles, when exposed to biological fluid, become coated with proteins and other biomolecules to form a 'protein corona'(1). Recent systematic studies have identified various proteins that can make up this corona, but these nanoparticle-protein interactions are still poorly understood, and quantitative studies to characterize them are few in number. Here, we have quantitatively analysed the adsorption of human serum albumin onto small (10-20 nm in diameter) polymer-coated FePt and CdSe/ZnS nanoparticles by using fluorescence correlation spectroscopy. The protein corona forms a monolayer with a thickness of 3.3 nm. Proteins bind to the negatively charged nanoparticles with micromolar affinity, and time-resolved fluorescence quenching experiments show that they reside on the particle for similar to 100 s. These new findings deepen our quantitative understanding of the protein corona, which is of utmost importance in the safe application of nanoscale objects in living organisms.