Modern mosaic analysis in the zebrafish
Modern mosaic analysis in the zebrafish
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DOI：
10.1016/j.ymeth.2006.02.002
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发表时间：
2006-07-01
期刊：
影响因子：
4.8
通讯作者：
Moens, Cecilia B.
中科院分区：
文献类型：
--
作者：
Carmany-Rampey, Amanda;Moens, Cecilia B.
用于深入了解复杂发育过程的最强大工具之一是嵌合胚胎的分析。嵌合体被定义为包含超过一种基因型的细胞的生物体，通常是野生型和突变型。嵌合体中野生型细胞和突变细胞之间的相互作用揭示了有关突变基因正常功能的信息。镶嵌分析已广泛应用于秀丽隐杆线虫、果蝇、小鼠和斑马鱼，以阐明基因在发育过程中何时、何地以及如何发挥作用。在斑马鱼中，镶嵌分析已被用来剖析许多不同的发育过程，包括原肠胚形成运动、中胚层和内胚层规范、神经元模式和迁移、轴突寻路、血管生成以及心脏、视网膜和神经嵴发育。镶嵌分析是了解斑马鱼基因功能的一种特别有效的方法，斑马鱼是一种特别适合向前遗传、分子和经典胚胎学方法的模式生物。这些属性与斑马鱼胚胎的可及性和光学清晰度相结合，有助于实时观察镶嵌胚胎内的单个细胞行为和相互作用。 (c) 2006 Elsevier Inc. 保留所有权利。
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of mosaic embryos. A mosaic is defined as an organism that contains cells of more than one genotype, usually wild-type and mutant. It is the interplay between wildtype and mutant cells in the mosaic that reveals information about the normal function of the mutated gene. Mosaic analysis has been utilized extensively in Caenorhabditis elegans, Drosophila, mice, and zebrafish to elucidate when, where, and how a gene acts during development. In the zebrafish, mosaic analysis has been used to dissect a number of different developmental processes, including gastrulation movements, mesoderm and endoderm specification, neuronal patterning and migration, axon pathfinding, angiogenesis, and cardiac, retinal, and neural crest development. Mosaic analysis is a particularly effective method for understanding gene function in the zebrafish, a model organism particularly suited to forward genetic, molecular, and classical embryological approaches. These attributes, when combined with the accessibility and optical clarity of the zebrafish embryo, facilitate the real time observation of individual cell behaviors and interactions within mosaic embryos. (c) 2006 Elsevier Inc. All rights reserved.