New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes
New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes
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DOI：
10.1016/j.mce.2012.05.013
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发表时间：
2012-10-15
影响因子：
4.1
通讯作者：
Takahashi, Takayuki
中科院分区：
文献类型：
--
作者：
Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
从青鳉卵巢中克隆了前列腺素E-2（PGE（2））受体亚型4，EP 4 b（Ptger 4 b）的cDNA。使用表达重组Ptger 4 b蛋白的COS-7细胞检测PGE（2）的作用。当细胞与PGE（2）孵育时，观察到细胞内cAMP水平增加，但加入EP 4拮抗剂GW 627368 X后，cAMP水平的增加被抵消。在体外培养的排卵前大卵泡中加入孕马血清促性腺激素可显著诱导ptger 4 b mRNA的表达。在GW 627368 X对卵泡排卵影响的体外排卵研究中，PGE（2）/Ptger 4 b相互作用的关键时间被认为是排卵的-1和0小时之间。这些结果进一步证实PGE（2）/Ptger 4 b信号通路参与青鳉卵巢排卵过程中卵泡破裂。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
A cDNA for a prostaglandin E-2 (PGE(2)) receptor subtype 4, EP4b (Ptger4b), was cloned from the medaka ovary. The effect of PGE(2) was examined using COS-7 cells expressing the recombinant Ptger4b protein. An increase in intracellular cAMP levels was observed when the cells were incubated with PGE(2), but the increase in cAMP levels was nullified by the addition of the EP4 antagonist GW627368X. The expression of ptger4b mRNA was drastically induced by the addition of pregnant mare serum gonadotropin to the in vitro culture of large preovulatory follicles. In in vitro ovulation studies of the effect of GW627368X addition on follicle ovulation, the critical timing of the PGE(2)/Ptger4b interaction was suggested to be between -1 and 0 h of ovulation. These results further substantiate that PGE(2)/Ptger4b signaling is involved in follicle rupture during ovulation in the medaka ovary. (C) 2012 Elsevier Ireland Ltd. All rights reserved.