Improved conditions for silver-ammonia staining of DNA in polyacrylamide gel
Improved conditions for silver-ammonia staining of DNA in polyacrylamide gel
复制标题
聚丙烯酰胺凝胶中 DNA 银氨染色条件的改进
DOI：
10.1002/elps.200900786
复制
发表时间：
2010-05-01
期刊：
影响因子：
2.9
通讯作者：
Li, Xiao-Kun
中科院分区：
文献类型：
--
作者：
Cong, Wei-Tao;He, Hong-Zhang;Li, Xiao-Kun
本文介绍了一种改进的聚丙烯酰胺凝胶DNA银氨染色法。在该方法中，使用银氨络合物的DNA染色允许高灵敏度、低成本、低毒性和简单的方案，而不需要固定和敏化步骤。该方法的检测限为1.5 pg，比传统的银氨染色法高出约30倍。与其他银染方法相比，该方法不仅对大片段DNA具有较高的灵敏度，而且对低碱基对DNA的检测也有更好的趋势。
An improved silver ammonia staining method for DNA on polyacrylamide gels is described. In this method, staining of DNA using silver ammonia complex allows high sensitivity, low cost, low toxicity, and simple protocol without requiring fixation and sensitization steps. The protocol takes less than 40 min to complete, with a detection limit of 1.5 pg of single DNA band on polyacrylamide gels, approximately 30-fold higher than that of original silver ammonia staining method. Furthermore, this novel technique not only exhibits high sensitivity for large DNA fragment, but also shows a better trend to detect low-base-pair DNA compared with other silver staining methods.