Th22, but not Th17 Might be a Good Index to Predict the Tissue Involvement of Systemic Lupus Erythematosus
Th22, but not Th17 Might be a Good Index to Predict the Tissue Involvement of Systemic Lupus Erythematosus
复制标题
DOI：
10.1007/s10875-013-9878-1
复制
发表时间：
2013-02
影响因子：
9.1
通讯作者：
Xuyan Yang;Hui‐ying Wang;Xiao-ying Zhao;Lijuan Wang;Qing-hua Lv;Qing-qing Wang
中科院分区：
文献类型：
--
作者：
Xuyan Yang;Hui‐ying Wang;Xiao-ying Zhao;Lijuan Wang;Qing-hua Lv;Qing-qing Wang
目的辅助性T细胞（Th）异常被认为与系统性红斑狼疮（SLE）的发病有关。Th 22细胞在系统性红斑狼疮（SLE）中的作用尚不清楚，但近年来Th 22细胞被发现参与了风湿性关节炎（RA）等自身免疫性疾病的发病机制。方法收集65例SLE患者和30例健康对照者的临床资料。将患者分为活动组和非活动组。通过流式细胞术测定CD 4 +IFN-γ−IL-17−IL-22+ T细胞（Th 22细胞）、CD 4 +IFN-γ−IL-22−IL-17+T细胞（Th 17细胞）和CD 4 +IFN-γ+（Th 1细胞）。血清白细胞介素-22（IL-22）和IL-17水平测定通过酶联免疫吸附试验（ELISA）。结果主要观察集中在增加的Th 22细胞在患者与唯一的狼疮皮肤病和减少的Th 22细胞在患者与唯一的狼疮肾炎。同样地，血清IL-22浓度在患有唯一性狼疮皮肤病的患者中升高，而在患有唯一性狼疮肾炎的患者中降低。此外，Th 22细胞的百分比与IL-22产生之间存在正相关。Th 17细胞百分比和血清IL-17浓度与系统性红斑狼疮疾病活动指数（SLEDAI）呈正相关。结论Th 22可能是一个比Th 17更有意义的指标，但Th 17可能在SLE活动中起作用。
PurposeT-helper (Th) cells abnormalities are considered to be associated with the pathogenesis of Systemic lupus erythematosus (SLE). Recently, The Th22 cells have been identified and implicated in the pathogenesis of autoimmune diseases such as Rheumatoid arthritis (RA), although therir role in Systemic lupus erythematosus (SLE) remains unclear. The present study intends to investigate their roles in SLE.MethodsClinical data were collected in 65 SLE patients and 30 healthy controls. The patients were divided into active and inactive groups. CD4+IFN-γ−IL-17−IL-22+Tcells (Th22 cells),CD4+IFN-γ−IL-22−IL-17+T cells (Th17 cells),and CD4+IFN-γ+(Th1 cells) were assayed by flow cytometry. Serum interleukin-22 (IL-22) and IL-17 levels were measured by enzyme-linked immunosorbent assay.ResultsThe main observation focused on increased Th22 cells in patients with sole lupus skin disease and decreased Th22 cells in patients with sole lupus nephritis. Likewise, concentrations of serum IL-22 were increased in patients with sole lupus skin disease, and decreased in patients with sole lupus nephritis. Additionally, there was a positive correlation between the percentage of Th22 cells and IL-22 production. The percentage of Th17 cells or concentration of serum IL-17 correlated positively with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI).ConclusionTh22 seems to be a more significant index to predict the tissue involvement of SLE than Th17, although Th17 may play a role in the activity of SLE.