Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Turkey: Occurrence of local topotype
Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Turkey: Occurrence of local topotype
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DOI：
10.1016/j.virusres.2009.12.014
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发表时间：
2010-04-01
期刊：
影响因子：
5
通讯作者：
Ozkul, Aykut
中科院分区：
文献类型：
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作者：
Ozkaya, Etem;Dincer, Ender;Ozkul, Aykut
本研究的目的是调查土耳其克里米亚-刚果出血热病毒（CCHFV）的分子流行病学。该研究对2006年至2008年共48例确诊的人类CCHF病例进行了研究。土耳其的大多数CCHF病毒株被发现属于欧洲血统。本地慢性充血性心力衰竭病毒株分为两个主要的集群，可进一步分为两个亚组。我们还确定了一种AP 92样病毒引起的临床疾病在科鲁姆（安纳托利亚中部省）。系统发育分析表明，最近的CCHFV感染所造成的内在（或本地）CCHF病毒株，我们确定为当地拓扑型。比较推导的S段RNA的氨基酸序列表明，当地的拓扑型来自前几年的病毒，最有可能是通过低速率重组。没有遗传差异，基于S-和M-节段RNA序列，发现人和蜱病毒分离株之间。这些数据表明，CCHFV在蜱载体中的复制，无论是扇头蜱属还是蜱属。或璃眼蜱属，对病毒基因组结构没有影响。(C)2010 Elsevier B. V.保留所有权利。
The goal of this study was to investigate the molecular epidemiology of Crimean-Congo hemorrhagic fever virus (CCHFV) in Turkey. The study was performed on a total of 48 confirmed human CCHF cases from 2006 to 2008. The majority of the CCHF viral strains in Turkey were found to belong to the European lineage. Local CCHF viral strains are grouped into two main clusters, which can be further divided into two sub-groups. We also identified an AP92-like virus causing clinical disease in Corum (a mid-Anatolian province). Phylogenetic analysis revealed that the most recent CCHFV infections were caused by intrinsic (or native) CCHF viral strains, which we identified as the local topotype. Comparison of deduced amino acid sequences of S-segment RNAs indicated that the local topotype was derived from viruses of previous years, most likely by a low rate recombination. No genetic differences, based on S- and M-segment RNA sequences, were found between human and tick viral isolates. This data suggest that replication of CCHFV in the tick vector, whether Rhiphicephalus spp. or Hyalomma spp., has no effect on the viral genomic structure. (C) 2010 Elsevier B.V. All rights reserved.