In vitro synthesis of proteoglycans associated with medullary bone in Japanese quail.
In vitro synthesis of proteoglycans associated with medullary bone in Japanese quail.
复制标题
日本鹌鹑髓质骨相关蛋白多糖的体外合成。
DOI：
10.1016/0003-9861(83)90410-1
复制
发表时间：
1983
影响因子：
3.9
通讯作者：
Schraer,H
中科院分区：
文献类型：
--
作者：
Hunter,SJ;Schraer,H
采用短期孵育系统研究了雌激素处理的雄性日本鹌鹑骨髓骨形成早期蛋白多糖的合成。蛋白聚糖通过在DEAEBio-GelA柱上用400-ml 0- 1 mNaCl梯度洗脱的色谱法分离。来自未注射的对照鸟的曲线显示出单峰，而来自雌激素处理的鸟的曲线显示出另一个峰的发展。[35 S]硫酸盐掺入雌激素诱导的蛋白多糖增加最显着之间25和37小时激素治疗后。雌激素诱导的蛋白聚糖在Sepharose CL-4 B上具有aKav= 0.65，平均浮力密度为1.50 μ g/ml，并且含有硫酸角质素作为其组分糖胺聚糖。第二种蛋白多糖在Sepharose CL-4 B上的aKav= 0.52，平均浮力密度≥ 1.7 g/ml，并以硫酸软骨素作为其主要糖胺聚糖。它也可能含有一些肝素或硫酸乙酰肝素。该结果支持了用于研究骨基质生产动力学的孵育系统的有用性。
A short-term incubation system was used to study proteoglycan synthesis during the early stages of medullary bone formation in estrogen-treated male Japanese quail. The proteoglycans were separated by chromatography on a DEAE Bio-Gel A column eluted with a 400-ml 0–1mNaCl gradient. The profile from uninjected control birds showed a single peak, whereas profiles from estrogen-treated birds showed development of another peak. Incorporation of [35S]sulfate into the estrogen-induced proteoglycan increased most dramatically between 25 and 37 h after hormone treatment. The estrogen-induced proteoglycan has aKav= 0.65 on Sepharose CL-4B, an average buoyant density of 1.50 g/ml, and contains keratan sulfate as its constituent glycosaminoglycan. The second proteoglycan has aKav= 0.52 on Sepharose CL-4B, an average buoyant density of ≥ 1.7 g/ml, and has chondroitin sulfate as it major glycosaminoglycan. It may also contain some heparin or heparan sulfate. The results support the usefulness of the incubation system for studying the dynamics of bone matrix production.