Lack of evidence for a role of renal tubular antigen in human membranous glomerulonephritis.
Lack of evidence for a role of renal tubular antigen in human membranous glomerulonephritis.
复制标题
缺乏证据证明肾小管抗原在人膜性肾小球肾炎中的作用。
作者：
Collins,AB;Andres,GA;McCluskey,RT
采用兔抗人肾小管抗原Fx1A的抗血清，通过间接免疫荧光法，对71例膜性肾小球肾炎患者的肾组织中肾小管抗原在肾小球沉积物中的存在进行了研究。兔抗血清与正常存在于人和大鼠肾脏近端小管刷状缘区域和Heymann肾炎大鼠肾小球沉积物中的抗原反应。然而，肾小管抗原检测不到任何人膜性肾小球肾炎的情况下，无论是之前或之后的免疫球蛋白从肾小球部分洗脱。5例患者的肾组织洗脱液和6例患者的血清不含免疫荧光法可证实的肾小管抗原抗体。这些发现未能提供肾小管抗原在人膜性肾小球肾炎中的作用的证据。
Renal tissue from 71 patients with membranous glomerulonephritis was studied for the presence of renal tubular antigens in glomerular deposits by indirect immunofluorescence, using a rabbit antiserum prepared against a human renal tubular antigen, Fx1A. The rabbit antiserum was reactive with antigens normally present in the brush border regions of proximal tubules of human and rat kidneys and in the glomerular deposits of rats with Heymann nephritis. However, renal tubular antigens were not detectable in any of the cases of human membranous glomerulonephritis, either before or after partial elution of immunoglobuhns from glomeruh. Eluates of renal tissue from 5 of the patients and sera from 6 patients did not contain antibodies against renal tubular antigens demonstrable by immunofluorescence. These findings fail to provide evidence for a role of renal tubular antigens in human membranous glomerulonephritis.