Heterogeneity in the metabolism of the arachidonoyl molecular species of glycerophospholipids of rabbit alveolar macrophages. The interrelationship between metabolic activities and chemical structures of the arachidonoyl molecular species.
Heterogeneity in the metabolism of the arachidonoyl molecular species of glycerophospholipids of rabbit alveolar macrophages. The interrelationship between metabolic activities and chemical structures of the arachidonoyl molecular species.
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兔肺泡巨噬细胞甘油磷脂花生四烯酰分子种类代谢的异质性。
DOI：
10.1111/j.1432-1033.1985.tb09296.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
K. Waku
中科院分区：
文献类型：
--
作者：
Y. Nakagawa;K. Kurihara;T. Sugiura;K. Waku
[3H]花生四烯酸 (20:4) 相对掺入兔肺泡巨噬细胞的二酰基和醚连接的甘油磷酸胆碱、甘油磷酸乙醇胺和甘油磷酸肌醇的 2 位含有 20:4 的单个分子种类（18:1-20:4、16:0-20:4 和 18:0-20:4 种类）中已通过反相测量高效液相色谱（HPLC）。 [3H]20:4 掺入甘油磷脂分子种类的速率很大程度上受其结构影响。反相HPLC分析通过比较具有相同结构（除了三种部分之一不同）的花生四烯酰分子种类的比放射性，可以阐明结构差异的影响，例如极性头基的性质、1位脂肪链和1位脂肪链键的化学形式对[3H]20:4摄取的影响。含有胆碱头基的分子种类的比放射性显着高于含有乙醇胺和肌醇部分的分子种类。二酰基分子的比放射性比醚连接的分子的高得多。连接在 1 位的脂肪链的性质也影响甘油磷脂对 [3H]20:4 的吸收。与二酰基或醚连接的甘油磷脂的相应 16:0-20:4 和 18:0-20:4 种类相比，在 1 位含有 18:1 的花生四烯酰分子种类优先用 [3H]20:4 标记。目前的结果表明，参与将20:4掺入甘油磷脂中的酰基转移酶对甘油磷脂的结构具有选择性，并且在本实验中推导出该酶对花生四烯酰分子种类的选择性顺序如下：胆碱头基大于乙醇胺和肌醇基团，酰基键大于醚和乙烯基醚键，18:1脂肪链大于16:0并且18:0 脂肪链位于 1 位。本文首次报道了具有单一结构的甘油磷脂的所有主要花生四烯酰分子种类的代谢活性的比较。
The relative incorporation of [3H]arachidonic acid (20:4) into individual molecular species containing 20:4 at the 2 position (18:1-20:4, 16:0-20:4 and 18:0-20:4 species) of diacyl and ether-linked glycerophosphocholine, glycerophosphoethanolamine and glycerophosphoinositol of rabbit alveolar macrophages has been measured by reversed-phase high-performance liquid chromatography (HPLC). The rate of incorporation of [3H]20:4 into the molecular species of glycerophospholipids was greatly influenced by their structures. The reversed-phase HPLC analysis allowed elucidation of the influence of structural differences, such as the nature of the polar head group, the fatty chain at the 1 position and the chemical form of the bond of the fatty chain attached at the 1 position on the uptake of [3H]20:4 by comparison of the specific radioactivities of arachidonoyl molecular species having the same structures, except that one of the three kinds of moiety was different. The specific radioactivities of the molecular species containing choline head groups were significantly higher than those containing ethanolamine and inositol moieties. The specific radioactivities of diacyl molecular species were considerably higher than those of ether-linked molecular species. The nature of the fatty chain attached at the 1 position also influenced the uptake of [3H]20:4 into glycerophospholipids. The arachidonoyl molecular species containing 18:1 at the 1 position were preferentially labelled with [3H]20:4 as compared to the corresponding 16:0-20:4 and 18:0-20:4 species either of diacyl or ether-linked glycerophospholipids. The present results suggest that the acyltransferase involved in the incorporation of 20:4 into glycerophospholipids has selectivity for the structures of glycerophospholipids and the order of selectivity of this enzyme for the arachidonoyl molecular species, deduced in the present experiments, was as follows: choline head group greater than ethanolamine and inositol groups, acyl bond greater than ether and vinyl ether bonds, 18:1 fatty chain greater than 16:0 and 18:0 fatty chains at the 1 position. Comparison of the metabolic activities of all major arachidonoyl molecular species of glycerophospholipids having a single structure is reported here for the first time.
单层培养的人子宫内膜基质细胞中花生四烯酸和前列腺素的代谢。
DOI：
10.1016/0005-2760(83)90272-2
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Korte,K;MacDonald,PC;Johnston,JM;Okita,JR;Casey,ML
通讯作者：
Casey,ML
离体豚鼠巨核细胞和血小板中花生四烯酸的合成和摄取。
DOI：
10.1016/0005-2760(84)90084-5
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Schick,PK;Schick,BP;Foster,K;Block,A
通讯作者：
Block,A
通过高效液相色谱分离烯基酰基、烷基酰基和二酰基类似物及其分子种类。
影响因子：
6.5
作者：
Nakagawa,Y;Horrocks,LA
通讯作者：
Horrocks,LA