AGPAT3 Gene polymorphisms are associated with milk production traits in Chinese Holstein cows
AGPAT3 Gene polymorphisms are associated with milk production traits in Chinese Holstein cows
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DOI：
10.1017/s0022029921000546
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发表时间：
2021-08-01
影响因子：
2.1
通讯作者：
Mao, Yongjiang
中科院分区：
文献类型：
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作者：
Sun, Xiaomei;Liang, Yan;Mao, Yongjiang
目前的研究报告鉴定了以前未发现的牛AGPAT3基因的单核苷酸多态性（SNPs），并进一步研究了它们与产奶量性状的关系。我们的研究结果表明，SNP g.12264的主要等位基因Cc> T与试验日产奶量、蛋白质率和305日产奶量呈正相关。重要的是，计算机分析表明，该位点的C/T转换产生了两个新的转录因子结合位点（TFBS）， E2F1和Nkx3-2。多态性g.18658gb>a是唯一与乳尿素氮（MUN）相关的SNP，其中G等位基因与乳尿素氮和脂肪率的增加有关。g.28731 SNP的GG基因型A > G与最高的脂肪和蛋白质百分比、最低的305天产奶量和体细胞评分（SCS）相关。AGPAT3位点与产奶量性状之间的关联可用于产奶量性状遗传改良的标记辅助选择，并可能与其他性状一起用于提高奶牛适应性的选择。
The current study reports the identification of previously undiscovered single-nucleotide polymorphisms (SNPs) in the bovine AGPAT3 gene and further investigates their associations with milk production traits. Our results demonstrate that the major allele C of the SNP g.12264 C > T is positively correlated with test-day milk yield, protein percentage and 305-day milk yield. Importantly, in silico analysis showed that the C/T transition at this locus gives rise to two new transcription factor binding sites (TFBS), E2F1 and Nkx3-2. Polymorphism g.18658 G > A was the only SNP associated with milk urea nitrogen (MUN) with the G allele related to an increase in milk urea nitrogen as well as fat percentage. The GG genotype of SNP g.28731 A > G was associated with the highest fat and protein percentage and lowest 305-day milk yield and somatic cell score (SCS). The association between AGPAT3 locus and milk production traits could be utilized in marker-assisted selection for the genetic improvement of milk production traits and, probably in conjunction with other traits, for selection to improve fitness of dairy cattle.