Effects of Salivary Gland Homogenate from Wild-Caught and Laboratory-Reared Lutzomyia longipalpis on the Evolution and Immunomodulation of Leishmania (Leishmania) amazonensis Infection
Effects of Salivary Gland Homogenate from Wild-Caught and Laboratory-Reared Lutzomyia longipalpis on the Evolution and Immunomodulation of Leishmania (Leishmania) amazonensis Infection
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DOI：
10.1111/j.1365-3083.2009.02310.x
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发表时间：
2009-10-01
影响因子：
3.7
通讯作者：
Pimenta, P. P. F.
中科院分区：
文献类型：
--
作者：
Laurenti, M. D.;da Matta, V. L. R.;Pimenta, P. P. F.
我们研究了野生捕获和实验室饲养载体的长须黄鼠唾液腺匀浆对亚马逊利什曼原虫（Leishmania）感染的病变演变和免疫调节的影响。为了比较两种唾液腺匀浆 (SGH) 的效果，在存在或不存在来自野生捕获和实验室定植白蛉的 SGH 的情况下，将 10(6) 个前鞭毛体皮下接种到 C57BL/6 小鼠的后足垫或耳真皮中。与SGH组相比，与野生捕获的SGH共接种的小鼠的病变大小较小，因为接种部位有巨噬细胞的寄生和浸润。与实验室饲养的 SGH 相比，野生捕获的 SGH 的 IL-4 和 IL-10 产量较低，但 IL-12 水平较高。我们的研究结果解决了在有关唾液效应的研究中使用实验室定植白蛉的 SGH 代替野生捕获的载体 SGH 可能存在的偏差。还推测白蛉唾液对利什曼原虫引起的自然感染可能有轻微影响，因为感染是通过野生媒介传播的，而不是通过实验室饲养的媒介传播的。
We investigated the effects of Lutzomyia longipalpis salivary glands homogenate of wild-caught and laboratory-reared vectors on the lesion evolution and immunomodulation of the infection caused by Leishmania (Leishmania) amazonensis. To compare the effect of both salivary glands homogenate (SGH), C57BL/6 mice were inoculated Subcutaneously into the hind footpads or into the ear dermis with 10(6) promastigotes in the presence or not of SGH from wild-caught and laboratory-colonized sand flies. Comparing SGH groups, the lesion size was lower in mice co-inoculated with wild-caught SGH, as the parasitism and the infiltration of macrophages at the inoculation site. Wild-caught SGH also determined lower production of IL-4 and IL-10 but higher IL-12 levels compared with laboratory-reared SGH. Our findings address a probable bias by using SGH from laboratory-colonized sand flies instead of wild-caught vector SGH in studies concerning saliva effects. A possible mild influence of sand fly saliva in natural infections caused by Leishmania is also speculated, as infection is transmitted by wild and not by laboratory-reared vectors.