Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana.
Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana.
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发表时间：
2000-11
期刊：
影响因子：
4.6
通讯作者：
E. Graaff;A. Dulk-Ras;P. Hooykaas;B. Keller
中科院分区：
文献类型：
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作者：
E. Graaff;A. Dulk-Ras;P. Hooykaas;B. Keller
在叶发育突变体的筛选中，我们已经分离出一个激活剂T-DNA标记的突变体，产生没有叶柄的叶子。除了多叶叶柄表型外，这种莴苣突变体还表现出异常的花序分枝和长角果形状。LEAFY PETIOLE（LEP）基因位于与这些显性表型连接的T-DNA插入片段的右边界附近，并编码具有与转录因子的AP 2/EREBP家族成员的DNA结合结构域相似的结构域的蛋白质。在野生型植物中引入激活标记的LEP基因赋予上述所有表型畸变。多叶叶柄表型由叶的近端部分从叶柄转化为叶片组成，这意味着叶的发育沿着近远轴受到干扰。因此，LEP参与边缘分生组织中的细胞分裂活动或沿近远轴沿着形成图案。
In a screen for leaf developmental mutants we have isolated an activator T-DNA-tagged mutant that produces leaves without a petiole. In addition to that leafy petiole phenotype this lettuce (let) mutant shows aberrant inflorescence branching and silique shape. The LEAFY PETIOLE (LEP) gene is located close to the right border of the T-DNA insert linked with these dominant phenotypes and encodes a protein with a domain with similarity to the DNA binding domain of members of the AP2/EREBP family of transcription factors. Introduction of the activation-tagged LEP gene in wild-type plants conferred all the phenotypic aberrations mentioned above. The leafy petiole phenotype consists of a conversion of the proximal part of the leaf from petiole into leaf blade, which means that leaf development in let is disturbed along the proximodistal axis. Therefore, LEP is involved in either cell division activity in the marginal meristem or patterning along the proximodistal axis.