EXPRESSION CLONING OF HUMAN EGF RECEPTOR COMPLEMENTARY-DNA - GENE AMPLIFICATION AND 3 RELATED MESSENGER-RNA PRODUCTS IN A431-CELLS
EXPRESSION CLONING OF HUMAN EGF RECEPTOR COMPLEMENTARY-DNA - GENE AMPLIFICATION AND 3 RELATED MESSENGER-RNA PRODUCTS IN A431-CELLS
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DOI：
10.1126/science.6326261
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
ROSENFELD, MG
中科院分区：
文献类型：
--
作者：
LIN, CR;CHEN, WS;ROSENFELD, MG
为了进一步明确多肽生长因子调控基因转录和细胞生长的机制，采用表达克隆技术筛选人表皮生长因子受体互补DNA克隆。egf3 '编码域与禽红母细胞病病毒（v-erbB）转化基因产物具有显著的同源性。在A431细胞系中，EGF受体的过表达与EGF受体mRNA水平的增加和明显单一的EGF受体基因的扩增（高达110倍）相关。在A431细胞中，EGF受体基因表达有三种胞质聚腺苷化RNA产物，其中一种仅含有5 ' （EGF结合域）序列，被认为编码分泌的EGF受体相关蛋白。
In order to further define the mechanisms by which polypeptide growth factors regulate gene transcription and cellular growth, expression cloning techniques were used to select human epidermal growth factor (EGF) receptor complementary DNA clones. The EGF 3′ coding domain shows striking homology to the transforming gene product of avian erythroblastosis virus (v-erbB). Over-expression of EGF receptors in A431 cell lines correlates with increased EGF receptor mRNA levels and amplification (up to 110 times) of the apparently singular EGF receptor gene. There appear to be three cytoplasmic polyadenylated RNA products of EGF receptor gene expression in A431 cells, one of which contains only 5′ (EGF binding domain) sequences and is postulated to encode the secreted EGF receptor-related protein.