Eluting trace components before major constituents. I: Sensitivity enhancement in analytical determinations of optical purity
Eluting trace components before major constituents. I: Sensitivity enhancement in analytical determinations of optical purity
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DOI：
10.1016/s0021-9673(01)94410-3
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发表时间：
1987
影响因子：
4.1
通讯作者：
J. A. Perry;J. D. Rateike;Ted Szczerba
中科院分区：
文献类型：
--
作者：
J. A. Perry;J. D. Rateike;Ted Szczerba
High optical purity can be precisely determined by high-performance liquid chromatography if several conditions can be met. Derivatization, if required, must be non-chiral; the elution order of enantiomers must be reversible, to allow elution of the trace enantiomer before the major enantiomer; and the column must under large overload retain efficiency for the trace enantiomer. Also for enhancing sensitivity to traces, we have found that a given quantity of sample is better injected in larger-than-conventional volume and at less-than-conventional concentration. Combining these approaches allowed not only the determination of 99.9967% optical purity in one demonstrative example but also an apparent extensibility to considerably higher purities. The limits to that extensibility, not obvious, were not established.
