Adipose tissue-derived mesenchymal stem cells acquire bone cell-like responsiveness to fluid shear stress on osteogenic stimulation
Adipose tissue-derived mesenchymal stem cells acquire bone cell-like responsiveness to fluid shear stress on osteogenic stimulation
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DOI：
10.1089/ten.2005.11.1780
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发表时间：
2005-11-01
影响因子：
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通讯作者：
Klein-Nulend, J
中科院分区：
文献类型：
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作者：
Knippenberg, M;Helder, MN;Klein-Nulend, J
为了设计骨组织，需要能够执行骨细胞特定功能的机械敏感细胞，例如骨组织的（重新）建模。在体内，局部骨量和结构受到机械负荷的影响，机械负荷被认为是通过负荷诱导的间质液流动激发细胞反应。脂肪组织是用于骨组织工程的间充质干细胞的易于获取的来源，并且与骨髓相比，其数量丰富。我们研究了脂肪组织源性间充质干细胞 (AT-MSC) 是否对体外成骨刺激的脉动流体流 (PFF) 的机械负荷有反应。我们发现，在 1,25-二羟基维生素 D-3 刺激成骨分化后，AT-MSC 对 PFF 导致的流体剪切应力表现出类似骨细胞的反应。在成骨刺激的 AT-MSC 中，PFF 增加一氧化氮的产生，并上调环氧合酶 2 的基因表达，但不上调环氧合酶 1 的基因表达。这些数据表明，AT-MSC 在 1,25-二羟基维生素 D-3 诱导的成骨分化中获得了对脉动流体剪切应力的骨细胞样反应。 AT-MSC 可能能够在体内骨（再）建模过程中执行骨细胞特异性功能，因此为骨组织工程提供了一种有前途的新工具。
To engineer bone tissue, mechanosensitive cells are needed that are able to perform bone cell-specific functions, such as ( re) modeling of bone tissue. In vivo, local bone mass and architecture are affected by mechanical loading, which is thought to provoke a cellular response via loading-induced flow of interstitial fluid. Adipose tissue is an easily accessible source of mesenchymal stem cells for bone tissue engineering, and is available in abundant amounts compared with bone marrow. We studied whether adipose tissue-derived mesenchymal stem cells (AT-MSCs) are responsive to mechanical loading by pulsating fluid flow (PFF) on osteogenic stimulation in vitro. We found that AT-MSCs show a bone cell-like response to fluid shear stress as a result of PFF after the stimulation of osteogenic differentiation by 1,25-dihydroxyvitamin D-3. PFF increased nitric oxide production, as well as upregulated cyclooxygenase-2, but not cyclooxygenase-1, gene expression in osteogenically stimulated AT-MSCs. These data suggest that AT-MSCs acquire bone cell-like responsiveness to pulsating fluid shear stress on 1,25-dihydroxyvitamin D-3-induced osteogenic differentiation. AT-MSCs might be able to perform bone cell-specific functions during bone ( re) modeling in vivo and, therefore, provide a promising new tool for bone tissue engineering.