Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study.
Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study.
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DOI：
10.1111/1469-0691.12134
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Smeesters PR
中科院分区：
文献类型：
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作者：
McMillan DJ;Drèze PA;Vu T;Bessen DE;Guglielmini J;Steer AC;Carapetis JR;Van Melderen L;Sriprakash KS;Smeesters PR
Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. were aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. 1086 isolates from 31 countries were analysed, representing 175 emm-types. emm-type is predictive of the whole protein structure, independent of geographic origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats, and predicted secondary structure was assessed in the context of the latest vaccine developments. Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80, M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.
