Tyrosine phosphorylation and activation of pp60c-src and pp125FAK in bradykinin-stimulated fibroblasts.
Tyrosine phosphorylation and activation of pp60c-src and pp125FAK in bradykinin-stimulated fibroblasts.
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缓激肽刺激的成纤维细胞中酪氨酸磷酸化以及 pp60c-src 和 pp125FAK 的激活。
DOI：
10.1152/ajpcell.1996.270.5.c1430
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Villereal,ML
中科院分区：
文献类型：
--
作者：
Lee,KM;Villereal,ML
缓激素（BK）刺激人包皮成纤维细胞（k - m）蛋白酪氨酸磷酸化。李，K. Toscas， M. L. Villereal， J.生物学。化学。268:9945-9948,1993)。酪氨酸磷酸化主要发生在分子质量为130和70 kDa的蛋白质中。在本报告中，我们证明了局灶黏附相关的酪氨酸激酶pp125FAK是130 kda磷酸酪氨酸带的一个组成部分。体外免疫复合物激酶测定表明，bk刺激的pp125FAK酪氨酸磷酸化水平与激酶活性增加密切相关。我们已经确定了paxillin，一种定位于局灶粘连的蛋白质，是70 kda磷酸酪氨酸带的一个组成部分。除了鉴定负责主要磷酸酪氨酸带的两种蛋白外，我们还报告pp60c-src在响应BK时被酪氨酸磷酸化和激活，分别通过免疫印迹和体外激酶分析。这些发现首次表明，BK受体与重要的原癌基因c-src偶联，并且src途径可能介导BK结合下游的一些事件。
Bradykinin (BK) stimulates protein tyrosine phosphorylation in human foreskin fibroblasts (K.-M. Lee, K. Toscas, and M. L. Villereal, J. Biol. Chem. 268:9945-9948, 1993). The major tyrosine phosphorylation occurs in proteins of a molecular mass of 130 and 70 kDa. In this report, we demonstrate that focal adhesion-associated tyrosine kinase, pp125FAK, is one component of the 130-kDa phosphotyrosine band. The BK-stimulated pp125FAK tyrosine phosphorylation level is well correlated with increased kinase activity, as assessed by in vitro immune complex kinase assays. We have identified paxillin, a protein that is localized in focal adhesions, as a component of the 70-kDa phosphotyrosine band. In addition to identifying the two proteins responsible for the major phosphotyrosine bands, we also report that pp60c-src is tyrosine phosphorylated and activated in response to BK, as analyzed by immunoblotting and in vitro kinase assays, respectively. These findings indicate, for the first time, that the BK receptor is coupled to the important protooncogene c-src and that the src pathway may mediate some of the events downstream from BK binding.
酪氨酸激酶抑制剂抑制缓激肽和毒胡萝卜素诱导的 Ca2+ 进入。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lee,KM;Toscas,K;Villereal,ML
通讯作者：
Villereal,ML