Regulation of indoleamine 2,3-dioxygenase activity in the small intestine and the epididymis of mice.
Regulation of indoleamine 2,3-dioxygenase activity in the small intestine and the epididymis of mice.
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DOI：
10.1016/0003-9861(80)90185-x
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发表时间：
1980-08
影响因子：
3.9
通讯作者：
R. Yoshida;T. Nukiwa;Yasuyoshi Watanabe;M. Fujiwara;F. Hirata;O. Hayaishi
中科院分区：
文献类型：
--
作者：
R. Yoshida;T. Nukiwa;Yasuyoshi Watanabe;M. Fujiwara;F. Hirata;O. Hayaishi
吲哚胺2，3-双加氧酶活性广泛分布于小鼠的脑、肺、胃、肠、附睾等组织中。消化道和附睾酶活性最高。以小肠和附睾液为材料，研究了吲哚胺2，3-双加氧酶活性的发育期和日节律性变化及各种调节因素的影响。在断奶的前2周，这两种组织中的酶活性都消失了。从第三周开始，活动迅速增加，在8到10周龄的小鼠(青春期)达到最大。小肠酶活性在30周龄或以后逐渐下降至零水平，而附睾酶活性在69周龄期间一直保持在较高水平(衰老)。饲喂时间在9：00-13：00的小鼠小肠酶活性呈昼夜节律变化，白天高，夜间低。夜间摄食(21：00-1：00)，酶活性夜间高，白天低。附睾酶活性在两种投喂方式下均无日变化。在发育和日常节律变化方面，小肠中吲哚胺2，3-双加氧酶的活性与肝脏色氨酸2，3-双加氧酶的活性相似。然而，与肝脏色氨酸2，3-双加氧酶活性相反，小肠和附睾吲哚胺2，3-双加氧酶活性不受肾上腺切除或肾上腺类固醇或色氨酸腹腔注射的影响。
Indoleamine 2,3-dioxygenase activity was found to be ubiquitously distributed in various tissues of mice, such as brain, lung, stomach, intestine, and epididymis. The highest enzyme activity was detected in the alimentary canal and the epididymis. Developmental and daily rhythmic changes of indoleamine 2,3-dioxygenase activity and the effects of various regulatory factors were studied with the supernatant fractions derived from the small intestine and the epididymis. The enzyme activity in these two tissues was absent during the first 2 weeks (the weaning period). From the third week, there was a rapid increase in activities and a maximum was reached when the mice were 8 to 10 weeks of age (adolescence). The enzyme activity in the small intestine then gradually diminished to zero level at 30 weeks of age (prime) or later, while that in the epididymis remained at the high level throughout 69 weeks of age (senescence). The enzyme activity of the small intestine from mice fed during the hours 9:00–13:00 showed daily rhythmic changes; high in the daytime and low at night. Under night feeding (21:00–1:00), the enzyme activity was high at night and low in the daytime. The epididymal enzyme activity showed no daily fluctuations by either feeding schedule. With regard to the developmental and daily rhythmic changes, indoleamine 2,3-dioxygenase activity in the small intestine was similar to that of hepatic tryptophan 2,3-dioxygenase. However, in contrast to the hepatic tryptophan 2,3-dioxygenase activity, indoleamine 2,3-dioxygenase activity in the small intestine and the epididymis was not affected by adrenalectomy or intraperitoneal administration of adrenal steroid or tryptophan.