Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells
Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells
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DOI：
10.1002/1873-3468.13325
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发表时间：
2019-02-01
期刊：
影响因子：
3.5
通讯作者：
Miyajima, Atsushi
中科院分区：
文献类型：
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作者：
Inagaki, Natsuko F.;Inagaki, Fuyuki F.;Miyajima, Atsushi
间皮细胞覆盖在哺乳动物的内脏器官和浆膜腔表面，在防止粘连中起着至关重要的作用。我们以前报道过，原代间皮祖细胞（MPCs）不仅可以防止术后粘连，而且可以促进肝切除术后的肝再生。诱导多能干细胞（iPSC）具有用于再生医学的潜力。在这里，我们已经建立了一个分化方案，小鼠iPSC衍生的MPC（miMPC）通过暴露于定义的因素，以及使用MPC特异性细胞表面抗原的纯化。此外，miMPCs具有抑制术后粘连和促进肝再生的能力。这是第一份报告强调功能性miMPCs的产生，这可能为从头细胞治疗提供潜力。
Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pluripotent stem cells (iPSCs) have the potential to be used for regenerative medicine. Here, we have established a differentiation protocol for mouse iPSC-derived MPCs (miMPCs) via the exposure to defined factors, as well as purification using MPC-specific cell surface antigens. Furthermore, the miMPCs had the ability to suppress postoperative adhesion and facilitate liver regeneration. This is the first report highlighting the generation of functional miMPCs, which may offer potential for de novo cell therapy.