Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome
Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome
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DOI：
10.1016/j.ijcard.2013.08.050
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发表时间：
2013-10-15
影响因子：
3.5
通讯作者：
Fu, Chunhui
中科院分区：
文献类型：
--
作者：
Lu, Yongguang;Li, Lang;Fu, Chunhui
背景：内皮微粒（Endothelial Microparticles，EMPs）参与炎症反应、凝血及血管功能调节。然而，电磁脉冲是否参与ACS的发病机制仍不清楚。方法：将85例急性心肌梗死（AMI）患者随机分为SAP组（n = 27）、UAP组（n = 28）和AMI组（n = 30）。20例冠状动脉造影正常的住院患者作为对照组。检测EMPs频率、IFN-γ和IL-4水平，并分析EMPs与Th 1/Th 2细胞因子的相关性。从ACS患者分离的PBMC在体外用EMP处理。采用流式细胞术检测Th 1/Th 2细胞计数，实时荧光定量PCR和Western blotting检测T-bet和加塔mRNA及蛋白表达，ELISA检测IFN-γ、TNF-α、IL-4和IL-10。EMPs与IFN-γ呈显著正相关。EMPs可通过增加T-bet mRNA和蛋白的表达，显著上调Th 1细胞的分化和功能。结论：EMP可能通过调节Th 1/Th 2的分化和功能，参与动脉粥样硬化的免疫和炎症过程。它们可能在冠状动脉粥样硬化和斑块不稳定的发病机制中起重要作用。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Background: Endothelial microparticles (EMPs) can be involved in inflammatory process, blood coagulation, and regulation of vascular function. However, it remains unclear whether EMPs participate in the pathogenesis of ACS. The purpose of this study is to investigate the impact of EMPs on Th1/Th2 development and functions in vitro.Methods: Eight-five patients were allocated into SAP group (n = 27), UAP group (n = 28), and AMI group (n = 30). Twenty hospitalized patients with normal coronary angiography were recruited as controls. The frequency of EMPs, IFN-gamma, and IL-4 levels were measured, and the correlation between EMPs and Th1/Th2 cytokine was analyzed. PBMCs isolated from patients with ACS were treated in vitro with EMPs. This was followed by flow cytometry for Th1/Th2 counts, real-time PCR and western blotting for T-bet and GATA mRNA and protein expression, and ELISA for IFN-gamma, TNF-alpha, IL-4, and IL-10.Results: This study proved that the frequency of EMPs was significantly increased in ACS patients. There was a significant positive correlation between EMPs and IFN-gamma. EMPs could significantly upregulate the differentiation and function of Th1 through increasing the expression of T-bet mRNA and protein. Furthermore, this study also indicated that EMP treatment in vitro could promote the expression of TNF-a, which exerts adverse effects on the pathogenesis and progression of atherosclerosis.Conclusions: EMPs may be involved in the immune and inflammatory processes that take part in artery atherosclerosis and that they do so by regulating Th1/Th2 differentiation and function. They may play an important role in the pathogenesis of coronary atherosclerosis and plaque instability. (C) 2013 Elsevier Ireland Ltd. All rights reserved.