Ancient origin of lubricated joints in bony vertebrates
Ancient origin of lubricated joints in bony vertebrates
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DOI：
10.7554/elife.16415
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发表时间：
2016-07-19
期刊：
影响因子：
7.7
通讯作者：
Crump, J. Gage
中科院分区：
文献类型：
--
作者：
Askary, Amjad;Smeeton, Joanna;Crump, J. Gage
滑膜关节是我们身体的骨骼之间的润滑连接，通常在关节炎中受到影响。一般认为，滑液关节最早是在脊椎动物来到陆地时进化出来的，而鳍鱼缺乏润滑关节。在这里，我们研究的表达和功能的哺乳动物滑膜关节，Prg 4/润滑蛋白，在不同的鳍鱼的关键润滑蛋白。我们发现，Prg 4同源物是专门丰富的下巴和胸鳍关节的斑马鱼，棘鱼，和gar，与斑马鱼prg 4 b基因的遗传缺失导致相同的年龄相关的关节退化中所看到的润滑素缺乏的小鼠和人类。我们的数据支持润滑滑膜关节的进化比目前公认的要早得多，至少在所有骨脊椎动物的共同祖先中是这样。在高度再生的斑马鱼中建立第一个关节炎模型将为了解滑膜关节疾病的病因和可能的治疗提供独特的机会。
Synovial joints are the lubricated connections between the bones of our body that are commonly affected in arthritis. It is assumed that synovial joints first evolved as vertebrates came to land, with ray-finned fishes lacking lubricated joints. Here, we examine the expression and function of a critical lubricating protein of mammalian synovial joints, Prg4/Lubricin, in diverse ray finned fishes. We find that Prg4 homologs are specifically enriched at the jaw and pectoral fin joints of zebrafish, stickleback, and gar, with genetic deletion of the zebrafish prg4b gene resulting in the same age-related degeneration of joints as seen in lubricin-deficient mice and humans. Our data support lubricated synovial joints evolving much earlier than currently accepted, at least in the common ancestor of all bony vertebrates. Establishment of the first arthritis model in the highly regenerative zebrafish will offer unique opportunities to understand the aetiology and possible treatment of synovial joint disease.