Expression of Thymidine Phosphorylase in Human Gastric Carcinoma
Expression of Thymidine Phosphorylase in Human Gastric Carcinoma
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胸苷磷酸化酶在人胃癌中的表达
DOI：
10.1111/j.1349-7006.1996.tb00219.x
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
T. Aikou
中科院分区：
文献类型：
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作者：
Y. Takebayashi;K. Miyadera;S. Akiyama;S. Hokita;Kazutaka Yamada;S. Akiba;Yuji Yamada;T. Sumizawa;T. Aikou
胸苷磷酸化酶（dThdR）的活性在几种恶性肿瘤中有增高的报道。实验证据表明，dThdlactone与血小板衍生的内皮细胞生长因子相同，并且dThdlactone具有血管生成活性。我们检测了胃癌组织中dThdR的表达，以探讨dThdR的表达是否与胃癌的血管生成、临床病理特征和预后有关。通过对内皮细胞进行VIII因子免疫染色来评估微血管。我们计数了158例肿瘤完全切除患者的肿瘤中的微血管。在新生血管形成最活跃的区域，在× 400视野中计数微血管。我们纯化了抗dThdR的单克隆抗体（TMA-1），并使用TMA-1在与用于检测因子VIII相同的连续切片中研究了dThdR的表达。探讨胃癌组织中血管生成与dThdR的关系及其临床病理意义。胃癌组织中dThdR阳性表达率（67/158，43.4%）明显高于正常胃组织（12/158，7.6%）（P<0.001）。dThd 3阳性胃癌的平均微血管计数高于dThd 3阴性胃癌（P<0.001）。胃癌细胞表达dThd 3的百分率与微血管计数呈显著正相关（P<0.001）。此外，dThd 7阳性癌的平均大小显著大于（P<0.001）阴性癌，dThd 7阳性胃癌的平均微血管计数也显著高于（P<0.001）dThd 7阴性癌。多因素Logistic回归分析显示，dThdR阳性表达与微血管计数（P<0.001）和淋巴结转移（P=0.013）相关。此外，总体上和III期患者中，dThd 3阳性癌患者的预后显著差于dThd 3阴性癌患者。这些结果表明，dThdR在胃癌中的表达与进展和转移有关，并且该酶影响某些疾病患者的预后。
The activity of thymidine phosphorylase (dThdPase) has heen reported to increase in several types of malignant tumors. Experimental evidence has shown that dThdPase is identical to platelet‐derived endothelial cell growth factor, and that dThdPase has angiogenic activity. We examined the expression of dThdPase to investigate whether the expression of dThdPase correlates with angiogenesis, clinicopathologic features and the prognosis of patients with human gastric carcinomas. Microvessels were assessed by immnnostaining endothelial cells for factor VIII. We counted microvessels in the tumors of 158 patients whose tumors were completely removed surgically. Microvessels were counted in a × 400 field in the most active areas of neovascularization. We purified a monoclonal antibody (TMA‐1) against dThdPase and studied the expression of dThdPase using TMA‐1 in the same serial sections as those used for the detection of factor VIII. The correlation between angiogenesis and dThdPase, and the clinicopathological significance of dThdPase, in patients with gastric carcinoma were examined. The positive expression of dThdPase was more frequent (P<0.001) in gastric carcinomas (67/158, 43.4%) than that in normal tissues (12/158, 7.6%). The average microvessel count in dThdPase‐positive gastric carcinomas was higher (P<0.001) than that in dThdPase‐negative carcinomas. The percentage of gastric carcinoma cells expressing dThdPase was significantly correlated with the microvessel count (P<0.001). Further, the average size of dThdPase‐positive carcinomas was significantly larger (P<0.001) than that of negative carcinomas and the mean microvessel count in dThdPase‐positive gastric carcinomas was also significantly higher (P<0.001) than that in dThdPase‐negative carcinomas. There was a significant correlation between the positive expression of dThdPase and microvessel count (P<0.001) or lymph node metastasis (P=0.013) by multivariate logistic analysis. Further, patients with dThdPase‐positive carcinoma showed a significantly worse prognosis than those with dThdPase‐negative carcinoma overall and in stage III. These findings indicate that the expression of dThdPase in gastric carcinomas is related to progression and metastasis, and this enzyme affects the prognosis of some patients with the disease.
Tatsuhiko Furukawa：“人胸苷磷酸化酶和血小板衍生的内皮细胞生长因子具有相同的序列。”
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