THE YEAST MATAL GENE CONTAINS 2 INTRONS
THE YEAST MATAL GENE CONTAINS 2 INTRONS
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DOI：
10.1002/j.1460-2075.1984.tb01927.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
MILLER, AM
中科院分区：
文献类型：
--
作者：
MILLER, AM
In the yeast S. cerevisiae there are 2 mating types, a and .alpha., which may mate to produce an a/.alpha. diploid. Mating type is determined by the allele (MATa or MAT.alpha.) occupying the MAT locus. In a diploid, expression of the MATa1 and MAT.alpha.2 genes determines the a/.alpha. state by regulating the expression of unlinked genes. Previous S1 endonuclease mapping implied that the MATa1 transcript is not processed. Further S1 mapping of this transcript was performed, demonstrating that the MATa1 gene contains 2 introns, unlike any other characterized nuclear gene in yeast. Both introns contain 5'' splice sites and 5''-TACTAACA-3'' consensus sequences at the positions predicted by the S1 mapping data. In the splicing-defective rna2 mutant, the mature message disappears rapidly and the precursor RNA accumulates. The RNA processing removes the UGA stop codon which was previously believed to be read-through.
