The early immune response to infection of chickens with Infectious Bronchitis Virus (IBV) in susceptible and resistant birds.
The early immune response to infection of chickens with Infectious Bronchitis Virus (IBV) in susceptible and resistant birds.
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DOI：
10.1186/s12917-015-0575-6
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发表时间：
2015-10-09
影响因子：
2.6
通讯作者：
Burt DW
中科院分区：
文献类型：
--
作者：
Smith J;Sadeyen JR;Cavanagh D;Kaiser P;Burt DW
传染性支气管炎是一种高度传染性的呼吸道疾病，它会导致气管病变，也会影响生殖道，每年给家禽业造成巨大的经济损失。这是由于死亡率（由IBV本身直接引起或由于随后的细菌感染）和产蛋量减少。这种病毒很难通过接种疫苗来控制，因此需要寻求新的方法来遏制这种疾病的影响。在这里，我们试图确定赋予对这种冠状病毒的抗性的基因，这可能有助于选择性育种计划，以培育不会屈服于这种疾病影响的鸡。应用全基因组基因表达芯片技术分析了鸡感染传染性支气管炎病毒（IBV）后基因表达的差异。在已知对病毒敏感或具有抗性的禽类中，在感染后2、3和4天检查对照和感染禽类的气管组织。在这3天内评价宿主先天免疫应答，并检查易感品系和抗性品系之间的差异。确定了参与IBV感染早期宿主反应的基因和生物学途径，并鉴定了易感和抗性鸟类之间的基因表达差异。突出了抗IBV的潜在候选基因。分析了宿主对IBV的早期反应，并鉴定了潜在的抗病候选基因。这些假定的抗性基因可用作未来遗传和功能研究的靶标，以证明与IBV抗性的因果联系。本文的在线版本（doi：10.1186/s12917-015-0575-6）包含补充材料，可供授权用户使用。
Infectious Bronchitis is a highly contagious respiratory disease which causes tracheal lesions and also affects the reproductive tract and is responsible for large economic losses to the poultry industry every year. This is due to both mortality (either directly provoked by IBV itself or due to subsequent bacterial infection) and lost egg production. The virus is difficult to control by vaccination, so new methods to curb the impact of the disease need to be sought. Here, we seek to identify genes conferring resistance to this coronavirus, which could help in selective breeding programs to rear chickens which do not succumb to the effects of this disease. Whole genome gene expression microarrays were used to analyse the gene expression differences, which occur upon infection of birds with Infectious Bronchitis Virus (IBV). Tracheal tissue was examined from control and infected birds at 2, 3 and 4 days post-infection in birds known to be either susceptible or resistant to the virus. The host innate immune response was evaluated over these 3 days and differences between the susceptible and resistant lines examined. Genes and biological pathways involved in the early host response to IBV infection were determined andgene expression differences between susceptible and resistant birds were identified. Potential candidate genes for resistance to IBV are highlighted. The early host response to IBV is analysed and potential candidate genes for disease resistance are identified. These putative resistance genes can be used as targets for future genetic and functional studies to prove a causative link with resistance to IBV. The online version of this article (doi:10.1186/s12917-015-0575-6) contains supplementary material, which is available to authorized users.