Pressure-driven sample injection with quantitative liquid dispensing for on-chip electrophoresis
Pressure-driven sample injection with quantitative liquid dispensing for on-chip electrophoresis
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DOI：
10.2116/analsci.20.483
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发表时间：
2004-03-01
影响因子：
1.6
通讯作者：
Seki, M
中科院分区：
文献类型：
--
作者：
Lee, NY;Yamada, M;Seki, M
开发了一种新的压力驱动进样方法作为电动进样的替代方法，并在采用该方法的微加工装置上进行了电泳分离。该方法能够实现限定体积的液体分配，然后通过气动压力驱动瞬时注射，大大简化了注射程序。一个特殊的微结构，称为“计量室”，已被设计用于定量分配的超低体积的样品液体;一个“疏水被动阀”配备了一个排气通道，用于注入分配的样品到分离通道。分配的重复性为3.3%（n = 15），由分配体积的变化表示。使用这种注射方法进行DNA片段的电泳分离，改变注射体积从0.45到4.0 nL，并比较分离效率。这种精确的进样方法，进样量容易变化，非常适合定量和定性电泳分析。
A novel pressure-driven sample injection method was developed as an alternative to electrokinetic injection, and electrophoretic separation was carried out on a microfabricated device employing this method. This method enables a defined volume of liquid dispensing, followed by instantaneous injection driven by pneumatic pressure, greatly simplifying the injection procedure. A particular microstructure, called a "metering chamber", has been designed for the quantitative dispensing of an ultra-low volume of sample liquid; a "hydrophobic passive valve" equipped with an air vent channel is employed for injecting a dispensed sample into the separation channel. The reproducibility of dispensing was 3.3% (n = 15), expressed by the variation of dispensed volumes. The electrophoretic separation of DNA fragments was performed using this injection method, varying the injection volumes from 0.45 to 4.0 nL, and the separation efficiencies were compared. This precise injection method, easily variable in injection volumes, is highly suitable for quantitative as well as qualitative electrophoretic analyses.