MOUSE EGG INTEGRIN ALPHA-6-BETA-1 FUNCTIONS AS A SPERM RECEPTOR
MOUSE EGG INTEGRIN ALPHA-6-BETA-1 FUNCTIONS AS A SPERM RECEPTOR
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DOI：
10.1016/s0092-8674(05)80014-5
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发表时间：
1995-06-30
期刊：
影响因子：
64.5
通讯作者：
WHITE, JM
中科院分区：
文献类型：
--
作者：
ALMEIDA, EAC;HUOVILA, APJ;WHITE, JM
精子和卵子质膜之间的结合是受精过程中的重要步骤。受精素是一种哺乳动物精子表面蛋白，参与了这种重要的相互作用，而卵质膜上的精子受体尚未被确定。由于受精素含有一个预测的整合素配体结构域，我们研究了整合素亚基在未受精小鼠卵子中的表达和功能。聚合酶链反应检测α 5、α 6、α v、β 1、β 3和β 5的mRNA。免疫荧光显示质膜上的α 6 β 1和α v β 3。GoH 3是一种功能阻断性抗α 6单克隆抗体，可消除精子结合，但非功能阻断性抗α 6单克隆抗体、功能阻断性抗α v β 3多克隆抗体和RGD肽则无影响。体细胞贪婪地结合精子，但只有当它们表达α 6 β 1时。受精素整合素配体结构域的肽类似物抑制精子与卵子和α 6 β 1（+）细胞的结合，并减少卵子的GoH 3染色。我们的研究结果表明整合素α 6 β 1作为细胞-细胞粘附受体介导精卵结合的新作用。
Binding between sperm and egg plasma membranes is an essential step in fertilization. Whereas fertilin, a mammalian sperm surface protein, is involved in this crucial interaction, sperm receptors on the egg plasma membrane have not been identified. Because fertilin contains a predicted integrin ligand domain, we investigated the expression and function of integrin subunits in unfertilized mouse eggs. Polymerase chain reactions detected mRNAs for alpha 5, alpha 6, alpha v, beta 1, beta 3, and beta 5. Immunofluorescence revealed alpha 6 beta 1 and alpha v beta 3 on the plasma membrane. GoH3, a function-blocking anti-alpha 6 monoclonal antibody, abolished sperm binding, but a nonfunction-blocking anti-alpha 6 monoclonal antibody, a function-blocking anti-alpha v beta 3 polyclonal antibody, and an RGD peptide had no effect. Somatic cells bound sperm avidly, but only if they expressed alpha 6 beta 1. A peptide analog of the fertilin integrin ligand domain inhibited sperm binding to eggs and alpha 6 beta 1(+) cells and diminished GoH3 staining of eggs. Our results indicate a novel role for the integrin alpha 6 beta 1 as a cell-cell adhesion receptor that mediates sperm-egg binding.