Hyperoxia improves oxygen consumption in the detached feline retina
Hyperoxia improves oxygen consumption in the detached feline retina
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DOI：
10.1167/iovs.06-0842
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发表时间：
2007-03-01
影响因子：
4.4
通讯作者：
Linsenmeier, Robert A.
中科院分区：
文献类型：
--
作者：
Wang, Shufan;Linsenmeier, Robert A.
目的.探讨高氧对视网膜脱离猫视网膜氧合和氧耗的影响。嗯-HODS。通过将0.25%透明质酸钠的平衡盐溶液注射到视网膜下腔中，在9只完整麻醉的猫中制造视网膜脱离。氧微电极用于收集视网膜PO 2的空间分布在附着和脱离的视网膜。结果视网膜脱离组视网膜与视网膜下液层交界处的PO 2降低，高氧组PO 2升高，与对照组（视网膜脱离，空气呼吸）相比无显著性差异。脱离并没有改变无血管和血管化视网膜之间的边界的PO 2;高氧显着增加的水平。在常氧期间，脱离视网膜的耗氧量降低至对照值的47% +/- 18%，Q（av）增加至对照值的68% +/- 17%。高氧使脱离的视网膜中的平均内部视网膜P02（PIR）增加至高于常氧期间的水平。在常氧条件下，分离并不改变P-IR。高氧已被证明可以改善视网膜脱离中的感光细胞存活。目前的工作表明，高氧是保护性的，因为它允许增加感光细胞的氧消耗。而正常的PO 2S维持在高氧期间的无血管区域的内部和外部边界，Qav没有恢复到正常，这表明除了氧输送不足之外，其他因素也参与了脱离期间的感光体功能障碍。
PURPOSE. To investigate the effects of hyperoxia on retinal oxygenation and oxygen consumption in the detached feline retina. MM-HODS. Retinal detachment was created in nine intact anesthetized cats by injecting 0.25% sodium hyaluronate in balanced salt solution into the subretinal space. Oxygen microelectrodes were used to collect spatial profiles of retinal PO2 in both the attached and detached retina. A diffusion model was fitted to quantify photoreceptor oxygen consumption (Q(av)).RESULTS In the detached retina, the PO2 at the border between the retina and the fluid layer under the retina decreased; hyperoxia increased it to a level that was not significantly different from the control (attached retina, air breathing). Detachment did not change the PO2 at the border between the avascular and vascularized retina; hyperoxia significantly increased the level. Oxygen consumption decreased to 47% +/- 18% of the control value in the detached retina during normoxia increased Q(av) to 68% +/- 17% of control. Hyperoxia increased the average inner retinal P02 (PIR) in the detached retina to a level higher than that during normoxia. Detachment did not change P-IR during normoxia.CONCLUSIONS. Hyperoxia has been shown to improve photoreceptor survival in the detached retina. The present work suggests that hyperoxia is protective because it allowed increased photoreceptor oxygen consumption. Whereas normal PO2S were maintained at the inner and outer border of the avascular region during hyperoxia, Qav was not restored to normal, suggesting that other factors are involved in photoreceptor dysfunction during detachment in addition to insufficient oxygen delivery.